Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (NGF-differentiated PC12 cells)
Specification
NGF-differentiated PC12 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Concentration
2 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit Alexa Fluor 488 (Molecular Probes
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
The cells were also stained with TOPRO-3 (Molecular Probes).
This anti-SOD2 antibody works as well as the polyclonal anti-SOD2 antibody, ab 13534
This anti-SOD2 antibody works as well as the polyclonal anti-SOD2 antibody, ab 13534
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Anne Chiaramello
Verified customer
提交于 Jun 17 2008