Anti-SOD2/MnSOD抗体(ab13533)
Key features and details
- Rabbit polyclonal to SOD2/MnSOD
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-SOD2/MnSOD抗体
参阅全部 SOD2/MnSOD 一抗 -
描述
兔多克隆抗体to SOD2/MnSOD -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Human -
免疫原
Recombinant fragment corresponding to Human SOD2/MnSOD.
Database link: P04179 -
阳性对照
- WB: Mouse Brain ; Rat Brain ; Rat Brain Membrane; Mouse Liver ; Mouse Hepatocytes
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was immunoaffinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13533于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (22) |
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa). |
靶标
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功能
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
疾病相关
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
序列相似性
Belongs to the iron/manganese superoxide dismutase family. -
翻译后修饰
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
- Unigene: 487046 Human
see all -
别名
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
图片
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All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/1000 dilution (o/n at 4degC)
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lane 3 : Rat brain membrane
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP for 1 hr at RT with shaking at 1/5000 dilution
Predicted band size: 26.6 kDaBlock: 5% Skim Milk powder in TBST.
Color Development: Chemiluminescent for HRP for 5 min at RT.
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All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : mouse liver whole cell lysate
Lane 2 : mouse hepatocytes whole cell lysate
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds
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All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution
Lane 1 : 2ug mouse liver homogenate supernatant
Lane 2 : 1ug mouse liver homogenate supernatant
Lane 3 : 0.5ug mouse liver homogenate supernatant
Secondary
All lanes : HRP conjugated donkey anti-rabbit antibody
Performed under reducing conditions.
Predicted band size: 26.6 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (301)
ab13533 被引用在 301 文献中.
- Brunetta HS et al. Nitrate consumption preserves HFD-induced skeletal muscle mitochondrial ADP sensitivity and lysine acetylation: A potential role for SIRT1. Redox Biol 52:102307 (2022). WB ; Mouse . PubMed: 35398714
- Lohakul J et al. Mitochondria-Targeted Hydrogen Sulfide Delivery Molecules Protect Against UVA-Induced Photoaging in Human Dermal Fibroblasts, and in Mouse Skin In Vivo. Antioxid Redox Signal 36:1268-1288 (2022). PubMed: 34235951
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Tripp TR et al. Time course and fibre type-dependent nature of calcium-handling protein responses to sprint interval exercise in human skeletal muscle. J Physiol 600:2897-2917 (2022). PubMed: 35556249
- Gong W et al. Orcinol Glucoside Improves Senile Osteoporosis through Attenuating Oxidative Stress and Autophagy of Osteoclast via Activating Nrf2/Keap1 and mTOR Signaling Pathway. Oxid Med Cell Longev 2022:5410377 (2022). PubMed: 35585885