概述

  • 产品名称
    Anti-SOD2/MnSOD抗体[9E2BD2]
    参阅全部 SOD2/MnSOD 一抗
  • 描述
    小鼠单克隆抗体[9E2BD2] to SOD2/MnSOD
  • 宿主
    Mouse
  • 经测试应用
    适用于: ICC/IF, IHC-P, IP, In-Cell ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Purified proteins from bovine liver, bovine heart, porcine heart

  • 阳性对照
    • Human HDFn and HL-60 cells and Human cerebellum.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab110300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. use 1mmol EDTA pH8
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 4 µg/ml. 0.4 µg/well
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • 疾病相关
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性
    Belongs to the iron/manganese superoxide dismutase family.
  • 翻译后修饰
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

图片

  • HL-60 cells were stained with 1 µg/mL ab110300 in blue or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry image of Superoxide Dismutase 2 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110300 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • Immunohistochemistry of Superoxide Dismutase 2 in Human cerebellum visualized with ab110300. Superoxide Dismutase 2 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of Superoxide Dismutase 2 immunoreactivity in the Purkinje cell bodies.

文献

This product has been referenced in:
  • González-Fernández C  et al. Wnt Signaling Alteration in the Spinal Cord of Amyotrophic Lateral Sclerosis Transgenic Mice: Special Focus on Frizzled-5 Cellular Expression Pattern. PLoS One 11:e0155867 (2016). IHC . Read more (PubMed: 27192435) »
  • Zhang C  et al. Heteroplasmic mutations of the mitochondrial genome cause paradoxical effects on mitochondrial functions. FASEB J : (2012). WB ; Human . Read more (PubMed: 22925728) »
See all 2 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The lab sent me the following information:

The customer should use this protocol: http://www.mitosciences.com/PDF/ip.pdf
As in this protocol we would recommend conjugating the antibody to the protein G beads prior to IP, but can also be done by adding beads to lysates/antibody mix. The detergent lauryl maltoside is ab109857 (https://www.abcam.com/10-Lauryl-Maltoside-Solution-ab109857.html)
We would recommend 1-5 mg lysates material from cell/tissue/mitochondria prepared using the detergent specific in the protocol (lauryl maltoside).



I hope this information is helpful to you. Please let me know if this protocol helps to get the IP to work with the endogenous samples.

Also, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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