概述

  • 产品名称
    Anti-SOCS3抗体
    参阅全部 SOCS3 一抗
  • 描述
    山羊多克隆抗体to SOCS3
  • 宿主
    Goat
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide: PIREFLDQYDAPL, corresponding to C terminal amino acids 213-225 of Human SOCS3 .

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • MOLT-4 lysate
  • 常规说明
    Principal Names - SOCS3; CIS3; Cish3; SSI-3; SOCS-3; suppressor of cytokine signaling 3; STAT induced STAT inhibitor 3; cytokine-induced SH2 protein 3 Official Gene Symbol - SOCS3 GenBank Accession Number – NP_003946 LocusLink ID - 9021 (human); 12702 (mouse); 89829 (rat) Gene Ontology terms - protein kinase inhibitor; JAK-STAT cascade; anti-apoptosis Size - 225 aa

性能

应用

Our Abpromise guarantee covers the use of ab723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Can be blocked with Human SOCS3 peptide (ab22776).
Approx 24kDa band seen in MOLT-4 lysate. Recommended for use at 4.0-6.0µg/ml. Block membrane in 2.5% skimmed milk in TBST (TBS + 0.05% Tween). Incubate primary antibody for 1 hour at room temperature with agitation.

 

靶标

  • 功能
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • 组织特异性
    Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • 通路
    Protein modification; protein ubiquitination.
  • 疾病相关
    Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • 序列相似性
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • 结构域
    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • 翻译后修饰
    Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all

图片

  • Western blot - Anti-SOCS3 antibody (ab723)
    Western blot - Anti-SOCS3 antibody (ab723)
    Anti-SOCS3 antibody (ab723) at 5 µg/ml + MOLT-4 lysate (RIPA buffer, 30µg total protein per lane)

    Observed band size: 24 kDa (why is the actual band size different from the predicted?)



    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

实验方案

文献

This product has been referenced in:
  • Minter MR  et al. Deletion of the type-1 interferon receptor in APPSWE/PS1?E9 mice preserves cognitive function and alters glial phenotype. Acta Neuropathol Commun 4:72 (2016). WB . Read more (PubMed: 27400725) »

See 1 Publication for this product

发表研究结果有使用 ab723？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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Western blot abreview for Anti-SOCS3 antibody

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Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (3)
Loading amount
45 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Dec 21 2017

We have decided to order the antibody SOCS 3 ( cat.no. ab723 ) and hopefully we have a good staining on the rat sections. Can you tell me the price in euro's ( + taxes )? Is there something known about the deliverytime?

Thank you for your email. Ab723 is 325 Euros and is in stock in our office in Great Britain so upon ordering, you should receive it within a couple of days. You can place your order online, or via fax, email or phone. Below is the appropriate contact d...

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We are interested in the SOCS 3 antibody ( cat.no. ab 723 ). I have some questions: Does this antibody work on rat material? We are using fresh or paraformaldehyde fixed rat brain tissue. The sections are vibratome sections and the technique is IHC.

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has no...

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Here is the answer to your questions. 1.High backgroung, several bands trouhgout the blot. The positive control did not show in the blot ( molt 4 whole cell lysate) 2.Human left ventricle, total protein 3.45ug of total protein. I used a 2% SDS lys...

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Thank you for the details that you have provided. We have had no other complaints concerning this antibody. The antibody's originator has provided me with the following protocol that they used when testing the antibody which should be helpful for you: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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