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Anti-SNX5抗体(ab5983)

Reviews (1)Q&A (2)References (5)
Western blot - Anti-SNX5 antibody (ab5983)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody (ab5983)
  • Western blot - Anti-SNX5 antibody (ab5983)

Key features and details

  • Goat polyclonal to SNX5
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-SNX5抗体
    参阅全部 SNX5 一抗

  • 描述

    山羊多克隆抗体to SNX5

  • 宿主

    Goat

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rat, Cow, Dog, Pig

  • 免疫原

    Synthetic peptide corresponding to Human SNX5 aa 350 to the C-terminus (C terminal). (NP_001269383.1)
    Database link: Q9Y5X3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Jurkat cell lysate. IHC-P: Human breast tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5983于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

1 hour primary incubation is recommended for this product.
说明
IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.

  • 序列相似性

    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.

  • 结构域

    The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.

  • 细胞定位

    Early endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 0910001N05Rik antibody
    • 1810032P22Rik antibody
    • AU019504 antibody
    • D2Ertd52e antibody
    • FLJ10931 antibody
    • OTTHUMP00000030340 antibody
    • OTTHUMP00000062927 antibody
    • OTTMUSP00000003367 antibody
    • OTTMUSP00000003368 antibody
    • RP11-504H3.2 antibody
    • RP23-35E16.2 antibody
    • snoRNA MBI-43 antibody
    • SNX 5 antibody
    • SNX5 antibody
    • SNX5_HUMAN antibody
    • Sorting nexin 5 antibody
    • Sorting nexin-5 antibody
    see all

图片

  • Western blot - Anti-SNX5 antibody (ab5983)
    Western blot - Anti-SNX5 antibody (ab5983)
    Anti-SNX5 antibody (ab5983) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    Primary incubated for 1 hour. Detected using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody (ab5983)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody (ab5983)
    ab5983 at 4µg/ml, staining SNX5 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and the sample was stained with HRP.
  • Western blot - Anti-SNX5 antibody (ab5983)
    Western blot - Anti-SNX5 antibody (ab5983)Image courtesy of an anonymous Abreview.
    Anti-SNX5 antibody (ab5983) at 1/500 dilution + whole cell lysate prepared from murine macrophages treated with INF gamma at 20 µg

    Secondary
    HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

文献 (5)

发表研究结果有使用 ab5983？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5983 被引用在 5 文献中.

  • Dong X  et al. Sorting nexin 5 mediates virus-induced autophagy and immunity. Nature 589:456-461 (2021). PubMed: 33328639
  • Cai J  et al. Sorting Nexin 5 Controls Head and Neck Squamous Cell Carcinoma Progression by Modulating FBW7. J Cancer 10:2942-2952 (2019). PubMed: 31281471
  • Halamoda-Kenzaoui B  et al. The agglomeration state of nanoparticles can influence the mechanism of their cellular internalisation. J Nanobiotechnology 15:48 (2017). WB ; Human . PubMed: 28651541
  • Steinberg F  et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . PubMed: 23563491
  • Esk C  et al. The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step. J Cell Biol 188:131-44 (2010). ICC/IF ; Human . PubMed: 20065094

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-SNX5 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage)
Loading amount
20 µg
Specification
macrophage
Treatment
IFNgamma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 01 2012

Question

Is it possible to purchase the blocking immunogen/peptide for this antibody, I cannot find an order number on the website.

Read More
Answer

Thank you for your enquiry. I'm afraid we don't sell the immunogen/peptide for this antibody. Please contact us again if you have further questions. Best of luck with your research.

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Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM High background, correct band size seen but many other bands. SAMPLE Human cell extracts PRIMARY ANTIBODY ABCAM AB5983, diluted 1 microg per ml of PBS-Tween with 3% BSA, incubated for 1 hour, washed 5x5 minutes with PBS-T DETECTION METHOD ECL plus POSITIVE AND NEGATIVE CONTROLS USED Jurlat cell lines used as a positive control, no negative control found ANTIBODY STORAGE CONDITIONS -80, not reconstituted, used as delivered SAMPLE PREPARATION Sample prepared with RIPA buffer and protease inhibitors, sample denatured with Laemmli buffer and BME AMOUNT OF PROTEIN LOADED 35 micro grams ELECTROPHORESIS/GEL CONDITIONS 8% separating gel with 6% stacking gel TRANSFER AND BLOCKING CONDITIONS Blocked with 5% milk initially, then Primary antibody (SNX5 with 3% BSA) and then secondary antibody with 3% BSA SECONDARY ANTIBODY Competitior's donkey anti-goat IgG-HRP, diluted X 20000, incubated for 30minutes to 1 hour HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Blocking reagents, 5% milk and times of incubation

Read More
Answer

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab5983. At this point I would like to make the following suggestions in order to help improve your results. Decrease the amount of secondary antibody that you are using and also make sure to run a secondary-only control to ensure that these additional bands are not due to your secondary (incubate with the primary only). Decrease the amount of primary antibody that you are using as well. Increase the amount of washes. Make sure that you block with 3% BSA rather than milk. Also, try boiling for 10 minutes rather than 5 minutes. If you need additional assistance, please contact us again.

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