Anti-SNX5抗体(ab5983)
Key features and details
- Goat polyclonal to SNX5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SNX5抗体
参阅全部 SNX5 一抗 -
描述
山羊多克隆抗体to SNX5 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide:
SLLQSCIDLFKNN
, corresponding to C terminal amino acids 392-404 of Human SNX5. -
常规说明
GenBank Accession Number – NP_055241; NP_689413Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 3 - 6 µg/ml. | |
WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation. -
序列相似性
Belongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain. -
结构域
The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate. -
细胞定位
Early endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation. - Information by UniProt
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数据库链接
- Entrez Gene: 514423 Cow
- Entrez Gene: 27131 Human
- Entrez Gene: 296199 Rat
- Omim: 605937 Human
- SwissProt: Q3ZBM5 Cow
- SwissProt: Q9Y5X3 Human
- SwissProt: B1H267 Rat
- Unigene: 316890 Human
see all -
别名
- 0910001N05Rik antibody
- 1810032P22Rik antibody
- AU019504 antibody
see all
图片
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Anti-SNX5 antibody (ab5983) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 47 kDa
Observed band size: 47 kDa
Primary incubated for 1 hour. Detected using chemiluminescence. -
ab5983 at 4µg/ml, staining SNX5 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and the sample was stained with HRP.
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Anti-SNX5 antibody (ab5983) at 1/500 dilution + whole cell lysate prepared from murine macrophages treated with INF gamma at 20 µg
Secondary
HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
实验方案
数据表及文件
文献 (4)
ab5983 被引用在 4 文献中.
- Cai J et al. Sorting Nexin 5 Controls Head and Neck Squamous Cell Carcinoma Progression by Modulating FBW7. J Cancer 10:2942-2952 (2019). PubMed: 31281471
- Halamoda-Kenzaoui B et al. The agglomeration state of nanoparticles can influence the mechanism of their cellular internalisation. J Nanobiotechnology 15:48 (2017). WB ; Human . PubMed: 28651541
- Steinberg F et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . PubMed: 23563491
- Esk C et al. The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step. J Cell Biol 188:131-44 (2010). ICC/IF ; Human . PubMed: 20065094