Anti-SNX5抗体(ab5983)
Key features and details
- Goat polyclonal to SNX5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SNX5抗体
参阅全部 SNX5 一抗 -
描述
山羊多克隆抗体to SNX5 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide:
SLLQSCIDLFKNN
, corresponding to C terminal amino acids 392-404 of Human SNX5. -
阳性对照
- WB: Jurkat cell lysate. IHC-P: Human breast tissue.
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常规说明
GenBank Accession Number – NP_055241; NP_689413The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation. -
序列相似性
Belongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain. -
结构域
The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate. -
细胞定位
Early endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation. - Information by UniProt
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数据库链接
- Entrez Gene: 514423 Cow
- Entrez Gene: 27131 Human
- Entrez Gene: 296199 Rat
- Omim: 605937 Human
- SwissProt: Q3ZBM5 Cow
- SwissProt: Q9Y5X3 Human
- SwissProt: B1H267 Rat
- Unigene: 316890 Human
see all -
别名
- 0910001N05Rik antibody
- 1810032P22Rik antibody
- AU019504 antibody
see all
图片
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Western blot - Anti-SNX5 antibody (ab5983)Anti-SNX5 antibody (ab5983) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 47 kDa
Observed band size: 47 kDa
Primary incubated for 1 hour. Detected using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody (ab5983)ab5983 at 4µg/ml, staining SNX5 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and the sample was stained with HRP. -
Western blot - Anti-SNX5 antibody (ab5983)Image courtesy of an anonymous Abreview.Anti-SNX5 antibody (ab5983) at 1/500 dilution + whole cell lysate prepared from murine macrophages treated with INF gamma at 20 µg
Secondary
HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab5983 被引用在 4 文献中.
- Cai J et al. Sorting Nexin 5 Controls Head and Neck Squamous Cell Carcinoma Progression by Modulating FBW7. J Cancer 10:2942-2952 (2019). PubMed: 31281471
- Halamoda-Kenzaoui B et al. The agglomeration state of nanoparticles can influence the mechanism of their cellular internalisation. J Nanobiotechnology 15:48 (2017). WB ; Human . PubMed: 28651541
- Steinberg F et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . PubMed: 23563491
- Esk C et al. The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step. J Cell Biol 188:131-44 (2010). ICC/IF ; Human . PubMed: 20065094