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Epigenetics and Nuclear Signaling Chromatin Remodeling Swi / Snf

Anti-SNF2H抗体- ChIP Grade (ab3749)

  • Datasheet
Reviews (9)Q&A (4)References (41)

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Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)
  • ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)

Key features and details

  • Rabbit polyclonal to SNF2H - ChIP Grade
  • Suitable for: ChIP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SNF2H抗体- ChIP Grade
    参阅全部 SNF2H 一抗
  • 描述

    兔多克隆抗体to SNF2H - ChIP Grade
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ChIP, WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human SNF2H aa 1000 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab5160)

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • BALB/3T3 whole cell lysate (ab7901)

应用

Our Abpromise guarantee covers the use of ab3749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use 2.5µl for 106 cells.
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).

靶标

  • 功能

    Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.
  • 组织特异性

    Ubiquitously expressed.
  • 序列相似性

    Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 SANT domains.
  • 发展阶段

    Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession O60264 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8467 Human
    • Entrez Gene: 93762 Mouse
    • Omim: 603375 Human
    • SwissProt: O60264 Human
    • SwissProt: Q91ZW3 Mouse
    • Unigene: 558422 Human
    • Unigene: 246803 Mouse
    • Unigene: 474173 Mouse
    • 别名

      • 4933427E24Rik antibody
      • D030040M08Rik antibody
      • D330027N15Rik antibody
      • EC 3.6.1.- antibody
      • hISWI antibody
      • hSNF2H antibody
      • ISWI antibody
      • MommeD4 antibody
      • SMARCA5 antibody
      • SMCA5_HUMAN antibody
      • Snf2h antibody
      • Sucrose nonfermenting like 5 antibody
      • Sucrose nonfermenting protein 2 homolog antibody
      • Sucrose nonfermenting, yeast, homolog of antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 antibody
      • WCRF135 antibody
      see all

    图片

    • Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)
      Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)

      Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.

      Lane 1: HeLa lysate (20ug)
      Lane 2: Mouse 3T3 lysate (20ug)
      Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
      Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
       
      Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)

      ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.

      Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.

      Lane 1: HeLa lysate (20ug)
      Lane 2: Mouse 3T3 lysate (20ug)
      Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
      Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug

      Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)

      ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immun
    • ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)
      ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)
       

      ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.

        

      Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro

    实验方案

    • ChIP protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (41)

    发表研究结果有使用 ab3749?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3749 被引用在 41 文献中.

    • Abeywickrama-Samarakoon N  et al. Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication. Nat Commun 11:419 (2020). PubMed: 31964889
    • Sabatella M  et al. ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31392348
    • Salvador Moreno N  et al. The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair. Nucleic Acids Res 47:2703-2715 (2019). PubMed: 30812030
    • Witwicki RM  et al. TRPS1 Is a Lineage-Specific Transcriptional Dependency in Breast Cancer. Cell Rep 25:1255-1267.e5 (2018). PubMed: 30380416
    • Min S  et al. Post-Translational Regulation of the RSF1 Chromatin Remodeler under DNA Damage. Mol Cells 41:127-133 (2018). WB . PubMed: 29385673
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 13 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SNF2H antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Human Cell (BPH1 prostate cells)
    Specification
    BPH1 prostate cells
    Permeabilization
    Yes - Triton 100
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 30 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SNF2H antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 04 2014

    Western blot abreview for Anti-SNF2H antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (12% SDS-PAGE)
    Sample
    Human Cell lysate - nuclear (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 05 2013

    Question

    I recently purchased the Anti-SNF2H antibody ab3749. For westerns, can I incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.1% Tween-20?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 10 2013

    Answer

    This would be an appropriate incubation solution for use with this antibody. We recommend use of this item at a starting concentration range near 1/500 and to incubate overnight at +4C.

    Read More

    Abcam Scientific Support

    回复于 Jun 10 2013

    Question

    Phone call: Customer kindly asked if they can get small sizes of antibodies before buying the whole product.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2011

    Answer

    Thank you for contacting us. As discussed on phone we unfortunately do not sell small sizes of our products. The reason is that; we have over 70,000 products in our catalogue and having the small sized sample of each would be difficult to keep. However in order to help our customers we have extended our Abpromise guarantee. As per our further conversation if you could get in touch again after selecting few products then I will certainly look into your request for further advice. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Oct 26 2011

    Western blot abreview for Anti-SNF2H antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (6%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 04 2011

    Immunoprecipitation abreview for Anti-SNF2H antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - nuclear (HeLa)
    Total protein in input
    200 µg
    Specification
    HeLa
    Immuno-precipitation step
    Protein A/G
    Read More

    Dr. Chee-Kwee Ea

    Verified customer

    提交于 Apr 28 2011

    Western blot abreview for Anti-SNF2H antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (osteosarcoma)
    Loading amount
    20 µg
    Specification
    osteosarcoma
    Treatment
    siRNA transfected with control and hsnf2h siRNA
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Sonia Rocha

    Verified customer

    提交于 Jul 17 2009

    Western blot abreview for Anti-SNF2H antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549)
    Loading amount
    100000 cells
    Specification
    A549
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 26 2008

    ChIP abreview for Anti-SNF2H antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Mouse Cell lysate - nuclear (Thymus, fibroblasts, hek293, jurkat)
    Specification
    Thymus, fibroblasts, hek293, jurkat
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Detection step
    Real-time PCR
    Positive control
    Thymus, fibroblasts, hek293, and jurkat cells
    The real time PCR primers amplified different regions of the hCD2 LCR
    Negative control
    Thymus, fibroblasts, hek293, and jurkat cells
    Primers amplifying the HPRT gene locus were used as a negative control
    Read More

    Dr. Eleni Ktisstaki

    Verified customer

    提交于 Nov 07 2006

    1-10 of 13 Abreviews or Q&A

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