Anti-SNF2H抗体- ChIP Grade (ab3749)
Key features and details
- Rabbit polyclonal to SNF2H - ChIP Grade
- Suitable for: IP, ICC/IF, ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SNF2H抗体- ChIP Grade
参阅全部 SNF2H 一抗 -
描述
兔多克隆抗体to SNF2H - ChIP Grade -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, ChIP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration. PubMed: 16917504
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
|
| ChIP | (1) |
Use 2.5µl for 106 cells.
|
| WB | (4) |
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. PubMed: 16917504 |
|
ICC/IF
Use at an assay dependent concentration. |
|
ChIP
Use 2.5µl for 106 cells. |
|
WB
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa). |
靶标
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功能
Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 SANT domains. -
发展阶段
Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8467 Human
- Entrez Gene: 93762 Mouse
- Omim: 603375 Human
- SwissProt: O60264 Human
- SwissProt: Q91ZW3 Mouse
- Unigene: 558422 Human
- Unigene: 246803 Mouse
- Unigene: 474173 Mouse
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别名
- 4933427E24Rik antibody
- D030040M08Rik antibody
- D330027N15Rik antibody
see all
图片
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Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)
ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immun -
ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro
数据表及文件
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Datasheet download
文献 (41)
ab3749 被引用在 41 文献中.
- Abeywickrama-Samarakoon N et al. Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication. Nat Commun 11:419 (2020). PubMed: 31964889
- Sabatella M et al. ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31392348
- Salvador Moreno N et al. The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair. Nucleic Acids Res 47:2703-2715 (2019). PubMed: 30812030
- Witwicki RM et al. TRPS1 Is a Lineage-Specific Transcriptional Dependency in Breast Cancer. Cell Rep 25:1255-1267.e5 (2018). PubMed: 30380416
- Min S et al. Post-Translational Regulation of the RSF1 Chromatin Remodeler under DNA Damage. Mol Cells 41:127-133 (2018). WB . PubMed: 29385673
