Anti-SNAP23抗体(ab4114)

Reviews (2)Q&A (3)References (18)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)

    Key features and details

    • Rabbit polyclonal to SNAP23
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-SNAP23抗体
      参阅全部 SNAP23 一抗

    • 描述

      兔多克隆抗体to SNAP23

    • 宿主

      Rabbit

    • 特异性

      This antibody reacts specifically with human SNAP23.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
      Database link: O00161

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4114于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

    • 组织特异性

      Ubiquitous. Highest levels where found in placenta.

    • 序列相似性

      Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.

    • 细胞定位

      Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • HsT17016 antibody
        • LS-B8340 antibody
        • SNAP 23 antibody
        • SNAP-23 antibody
        • SNAP23 antibody
        • SNAP23A antibody
        • SNAP23B antibody
        • SNP23_HUMAN antibody
        • Synaptosomal associated protein 23 antibody
        • Synaptosomal associated protein 23kDa antibody
        • Synaptosomal associated protein antibody
        • Synaptosomal-associated protein 23 antibody
        • Vesicle membrane fusion protein SNAP 23 antibody
        • Vesicle membrane fusion protein SNAP23 antibody
        • Vesicle-membrane fusion protein SNAP-23 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)

        IHC image of ab4114 staining in human placenta tissue formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      文献 (18)

      发表研究结果有使用 ab4114？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab4114 被引用在 18 文献中.

      • Xiong X  et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470
      • Stévenin V  et al. Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche. Cell Rep 29:3958-3973.e7 (2019). PubMed: 31851926
      • Deng B  et al. Plasma membrane cholesterol trafficking in steroidogenesis. FASEB J 33:1389-1400 (2019). PubMed: 30133326
      • Bertuccio CA  et al. Plasma membrane insertion of KCa2.3 (SK3) is dependent upon the SNARE proteins, syntaxin-4 and SNAP23. PLoS One 13:e0196717 (2018). PubMed: 29768434
      • Lesteberg K  et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Flow Cyt ; Human . PubMed: 28822075
      View all Publications for this product

      客户评价及客户问答

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      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-SNAP23 antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Lung)
      Loading amount
      20 µg
      Specification
      Lung
      Gel Running Conditions
      Reduced Denaturing (12)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
      Read More

      Mrs. Katelyn Miller

      Verified customer

      提交于 Jun 25 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SNAP23 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (neurospheres)
      Specification
      neurospheres
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - triton-x
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      提交于 Sep 05 2007

      Question

      I was concerned about cross-reactivity with mouse or other rodent SNAP23 proteins. Since we are working with the mouse this information would be very valuable before getting this reagent. Is an other way would consist to test ourselves if you were open to send us a small aliquot?

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      Answer

      I'm sorry to inform you have we have no data for the cross reactivity of this anitbody with mouse or other rodent synaptosome associated protein 23. I am not familiar with the degree of sequence homology between human and murine SNAP23. However, as the antibody is a polyclonal it will recognise multiple epitopes across SNAP23 and is therefore more likely to cross react with the protein from different species. If you are still interested in this antibody, please contact me directly. Danielle N. Miller PhD Head of Customer Services danielle.miller@abcam.com

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      Question

      Is there a positive control available for use in WB with this antibody?

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      Answer

      No, sorry. The only control is with the endogenous protein from the cell line described in the data sheet.

      Read More

      Question

      Dear Sir anti snap-23 reacts human SNAP-23, how about rat or mouse? I want to apply against rat homogenate.

      Read More
      Answer

      This antibody has not yet been tested for cross-reactivity against rat or mouse SNAP-23.

      Read More

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