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Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs

Anti-SNAP23抗体(ab3340)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (13)

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Western blot - Anti-SNAP23 antibody (ab3340)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab3340)
  • Western blot - Anti-SNAP23 antibody (ab3340)
  • Western blot - Anti-SNAP23 antibody (ab3340)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
  • Western blot - Anti-SNAP23 antibody (ab3340)

Key features and details

  • Rabbit polyclonal to SNAP23
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Rat, Human, Recombinant fragment
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SNAP23抗体
    参阅全部 SNAP23 一抗
  • 描述

    兔多克隆抗体to SNAP23
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Rat, Human, Recombinant fragment
  • 免疫原

    Synthetic peptide corresponding to Mouse SNAP23 aa 193-210.
    Sequence:

    NKNRIDIANTRAKKLIDS


    (Peptide available as ab4956)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SNAP23 protein (ab79180)

应用

Our Abpromise guarantee covers the use of ab3340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. 23kDa band represents SNAP 23 from rat brain protein extract.
ICC/IF Use a concentration of 2 µg/ml.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能

    Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
  • 组织特异性

    Ubiquitous. Highest levels where found in placenta.
  • 序列相似性

    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • 细胞定位

    Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
  • Target information above from: UniProt accession O00161 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8773 Human
    • Entrez Gene: 64630 Rat
    • Omim: 602534 Human
    • SwissProt: O00161 Human
    • SwissProt: O70377 Rat
    • Unigene: 511149 Human
    • Unigene: 14789 Rat
    • 别名

      • HsT17016 antibody
      • LS-B8340 antibody
      • SNAP 23 antibody
      • SNAP-23 antibody
      • SNAP23 antibody
      • SNAP23A antibody
      • SNAP23B antibody
      • SNP23_HUMAN antibody
      • Synaptosomal associated protein 23 antibody
      • Synaptosomal associated protein 23kDa antibody
      • Synaptosomal associated protein antibody
      • Synaptosomal-associated protein 23 antibody
      • Vesicle membrane fusion protein SNAP 23 antibody
      • Vesicle membrane fusion protein SNAP23 antibody
      • Vesicle-membrane fusion protein SNAP-23 antibody
      see all

    图片

    • Western blot - Anti-SNAP23 antibody (ab3340)
      Western blot - Anti-SNAP23 antibody (ab3340)
      All lanes : Anti-SNAP23 antibody (ab3340) at 1 µg/ml

      Lane 1 : Untransfected U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates
      Lane 2 : Non-specific scrambled siRNA transfected U-87 MG whole cell lysates
      Lane 3 : SNAP23 KO U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/ml


      Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SNAP23.

    • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
      Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)

      Immunofluorescence analysis of SNAP-23 was performed using 90% confluent log phase U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells with ab3340. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3340 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab3340)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab3340)
      ab3340 (1ug/ml) staining SNAP23 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartments of the lymphatic nodules.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Western blot - Anti-SNAP23 antibody (ab3340)
      Western blot - Anti-SNAP23 antibody (ab3340)
      All lanes : Anti-SNAP23 antibody (ab3340) at 2 µg/ml

      Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg/ml per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.4 µg/ml
    • Western blot - Anti-SNAP23 antibody (ab3340)
      Western blot - Anti-SNAP23 antibody (ab3340)
      Anti-SNAP23 antibody (ab3340) + Rat brain tissue
    • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
      Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)

      ICC/IF image of ab3340 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-SNAP23 antibody (ab3340)
      Western blot - Anti-SNAP23 antibody (ab3340)
      Anti-SNAP23 antibody (ab3340) at 1 µg/ml + Recombinant Human SNAP23 protein (ab79180) at 0.001 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 2 minutes

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (13)

    发表研究结果有使用 ab3340?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3340 被引用在 13 文献中.

    • Yang Y  et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
    • Søgaard D  et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
    • Qiu H  et al. Inhibiting Notch1 enhances immunotherapy efficacy in melanoma by preventing Notch1 dependent immune suppressive properties. Cancer Lett 434:144-151 (2018). PubMed: 30036609
    • Wei Y  et al. Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23. Nat Commun 8:14041 (2017). PubMed: 28067230
    • Yang H  et al. Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice. Neural Plast 2017:4526417 (2017). PubMed: 29318050
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-SNAP23 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (COS7)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    COS7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 20 2015

    Western blot abreview for Anti-SNAP23 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Madin-Darby canine kidney (MDCK) cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Madin-Darby canine kidney (MDCK) cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 02 2015

    Western blot abreview for Anti-SNAP23 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Bovine aortic endothelial cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Bovine aortic endothelial cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 26 2015

    Western blot abreview for Anti-SNAP23 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (RBL-2H3)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    RBL-2H3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 08 2015

    Western blot abreview for Anti-SNAP23 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HEK293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 30 2015

    Western blot abreview for Anti-SNAP23 antibody

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    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (J774 macrophages)
    Specification
    J774 macrophages
    Treatment
    siRNA for 48h
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 28 2014

    Immunoprecipitation abreview for Anti-SNAP23 antibody

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    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3)
    Total protein in input
    1e+006 cells
    Specification
    NIH 3T3
    Immuno-precipitation step
    Other
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 22 2006

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