Key features and details
- Rabbit polyclonal to SNAIL + SLUG
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
存放说明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab180714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/50 - 1/200.|
相关性Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
细胞定位Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
Anti-SNAIL + SLUG antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts
Predicted band size: 29 kDa
All lanes : Anti-SNAIL + SLUG antibody (ab180714)
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Rat liver tissue lysate
Predicted band size: 29 kDa
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SNAI1+SLUG with ab180714 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling SNAIL + SLUG with ab180714 at 1/100 dilution.
Western blot analysis of extracts of various cell lines using ab180714 at the dilution 1/500.
ab180714 staining SNAIL in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a basic buffer (TRIS+EDTA, pH9). Samples were incubated with primary antibody (1/500 in antibody diluent) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SNAIL +SLUG with ab180714 at 1/100 dilution.
Lane 1: Snail recombinant 0.1 ug (55 kDa)
Lane 2: Slug recombinant 0.1 ug (56 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.
ab180714 被引用在 101 文献中.
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- Du X et al. LINC00511 contributes to glioblastoma tumorigenesis and epithelial-mesenchymal transition via LINC00511/miR-524-5p/YB1/ZEB1 positive feedback loop. J Cell Mol Med 24:1474-1487 (2020). PubMed: 31856394
- Liu YP et al. circPVT1 Facilitates Invasion and Metastasis by Regulating miR-205-5p/c-FLIP Axis in Osteosarcoma. Cancer Manag Res 12:1229-1240 (2020). PubMed: 32110097