概述

  • 产品名称
    Anti-smooth muscle Myosin heavy chain 11抗体[1G12]
    参阅全部 smooth muscle Myosin heavy chain 11 一抗
  • 描述
    小鼠单克隆抗体[1G12] to smooth muscle Myosin heavy chain 11
  • 宿主
    Mouse
  • 特异性
    This antibody reacts with human, mouse, and rat smooth muscle myosin heavy chain 11 (SM2). Does not cross-react with smooth muscle Myosin heavy chain 1.
  • 经测试应用
    适用于: WB, IHC-Fr, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11. The immunogen sequence exists in isoforms 3 and 4 but not isoforms 1 and 2 of the UniProt record P35749.
    Sequence:

    RGPPPQETSQ


    Database link: P35749-3

性能

应用

Our Abpromise guarantee covers the use of ab683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. PubMed: 21103378
IHC-Fr 1/400.
ICC/IF 1/250.
IHC-P 1/400. For paraffin embedded sections, Carnoy's fixative may give better results than formalin.

靶标

  • 功能
    Muscle contraction.
  • 组织特异性
    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • 疾病相关
    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • 序列相似性
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 结构域
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 细胞定位
    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

图片

  • ab683 at 1/400 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa Fluor® 594 conjugated goat anti-mouse IgG1 was used as the secondary.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain 11 in rat cells from cells from mesenteric artery by ICC/IF. Cells were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 2% serum for 30 minutes at 20°C. The primary antibody was diluted 1/300 and incubated with the sample for 14 hours at 4°C. Alexa Fluor® 488 chicken polyclonal to mouse Ig, diluted 1/400, was used as the secondary. Nuclei stained with Hoechst 33342.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain 11 in human cerebral artery tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton-X and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with the primary antibody (1/500) for 16 hours at 4°C. A biotin-conjugated rabbit anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain in human freshly isolated arterial myocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 30 minutes at 20°C. Samples were incubated with the primary antibody (1/300 in PBS) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated chicken anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Li Z  et al. Upregulation of Oxytocin Receptor in the Hyperplastic Prostate. Front Endocrinol (Lausanne) 9:403 (2018). Read more (PubMed: 30123183) »
  • Zhou B  et al. Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury. Arterioscler Thromb Vasc Biol 37:675-684 (2017). Read more (PubMed: 28153879) »
See all 29 Publications for this product

客户评价及客户问答

1-10 of 36 Abreviews or Q&A

Question
Answer

Thank you for contacting Abcam.

Please find links below to a few antibodies that we have that recognize smooth muscle heavy chain 1 and 2:

https://www.abcam.com/Myosin-smooth-muscle-heavy-chain-1-and-2-antibody-EPR5335-ab124679.html
https://www.abcam.com/Myosin-smooth-muscle-heavy-chain-1-and-2-antibody-V-10-ab77967.html



Since neither, of these antibodies have been tested in either human and mouse or validated in ICC/IF on cultured cells, you may be able to take advantage of out testing discount program:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



Please let me know if there is anything else I can help you with or if you would like to take advantage of the testing discount program.

Read More

Answer

Thank you for contacting us.

The antibody ab683 will recognize SM2A and SM2B:

Human SM2A

http://www.ncbi.nlm.nih.gov/protein/13124875?report=genbank&log$=prottop&blast_rank=8&RID=R93GH1VT016

Human SM2B

http://www.ncbi.nlm.nih.gov/protein/92091583?report=genbank&log$=prottop&blast_rank=10&RID=R93GH1VT016

It will not recognize SM1A or SM1B, but it cannot distinguish between the A and B isoforms of SM2.

For an antibody specific for SM1, we have ab682. This will not react with SM2, but it cannot distinguish between the A and B isoforms of SM1.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

Yes, I'd be happy to replace ab683 with ab53219 (which is also an Anti-smooth muscle Myosin heavy chain 11 antibody). Since this antibody has not been tested in human samples, we would not be able to guarantee it for human samples. Thus, the Abpromise guarantee would not apply. However, I can issue a testing discount code for the customer. For more details on this offer, please see below.



For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


Please let me know if the customer would like to receive the testing discount code.


I look forward to hear back from you and assist you further.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Carotid Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization
Yes - 0.1% Triton X-100 for 15 mins
Specification
Carotid Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Oct 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Temporal Artery Biopsy)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Specification
Temporal Artery Biopsy
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

April Rempel

Verified customer

提交于 Apr 30 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Sample
Human Cell (Human freshly isolated arterial myocyte)
Specification
Human freshly isolated arterial myocyte
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Mar 31 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Sample
Mouse Cultured Cells (primary vascular smooth muscle cell)
Specification
primary vascular smooth muscle cell
Permeabilization
Yes - 0.25% Triton-X100
Fixative
Formaldehyde

Mr. Mc Shen

Verified customer

提交于 Jan 29 2015

Answer



Les anticorps ab682 et ab683 ne sont pas purifié et donc la concentration n’a pas été déterminée. Ces anticorps sont des ascites et nous estimons les concentrations d’être entre 5-10 mg/ml.

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Question
Answer

Thank you for contacting us.

Please find attached protocol for ab7817.

The IHC-P protocol for ab683 can be found in publication;

http://ops.adminsite.com/admin/abcamshare/CRM/attach/2013/3/1/CCE4718082_img-301091703.pdf

The protocol for ab32575 can be found at the link http://www.epitomics.com/products/support/ihc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

Your credit note ID for the refund is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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1-10 of 36 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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