Anti-SMN/Gemin 1抗体[2B1] (ab5831)

Reviews (3)Q&A (5)References (16)
Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
  • Western blot - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
  • Immunoprecipitation - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
  • Flow Cytometry - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
  • Western blot - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)

Key features and details

  • Mouse monoclonal [2B1] to SMN/Gemin 1
  • Suitable for: IHC-P, ICC/IF, WB, IP, Flow Cyt
  • Reacts with: Human, Xenopus laevis
  • Isotype: IgG1

概述

  • 产品名称

    Anti-SMN/Gemin 1抗体[2B1]
    参阅全部 SMN/Gemin 1 一抗

  • 描述

    小鼠单克隆抗体[2B1] to SMN/Gemin 1

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, ICC/IF, WB, IP, Flow Cytmore details

  • 种属反应性

    与反应: Human, Xenopus laevis

  • 免疫原

    Recombinant full length protein corresponding to Human SMN/Gemin 1.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5831于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (2)
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
说明
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能

    The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.

  • 组织特异性

    Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level).

  • 疾病相关

    Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 1 (SMA1) [MIM:253300]. Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. Autosomal recessive forms are classified according to the age of onset, the maximum muscular activity achieved, and survivorship. The severity of the disease is mainly determined by the copy number of SMN2, a copy gene which predominantly produces exon 7-skipped transcripts and only low amount of full-length transcripts that encode for a protein identical to SMN1. Only about 4% of SMA patients bear one SMN1 copy with an intragenic mutation. SMA1 is a severe form, with onset before 6 months of age. SMA1 patients never achieve the ability to sit.
    Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 2 (SMA2) [MIM:253550]. SMA2 is an autosomal recessive spinal muscular atrophy of intermediate severity, with onset between 6 and 18 months. Patients do not reach the motor milestone of standing, and survive into adulthood.
    Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 3 (SMA3) [MIM:253400]. SMA3 is an autosomal recessive spinal muscular atrophy with onset after 18 months. SMA3 patients develop ability to stand and walk and survive into adulthood.
    Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 4 (SMA4) [MIM:271150]. SMA4 is an autosomal recessive spinal muscular atrophy characterized by symmetric proximal muscle weakness with onset in adulthood and slow disease progression. SMA4 patients can stand and walk.

  • 序列相似性

    Belongs to the SMN family.
    Contains 1 Tudor domain.

  • 细胞定位

    Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
  • Information by UniProt

  • 数据库链接

    • 别名

      • BCD541 antibody
      • Component of gems 1 antibody
      • Gemin 1 antibody
      • Gemin-1 antibody
      • OTTHUMP00000125198 antibody
      • OTTHUMP00000223567 antibody
      • OTTHUMP00000223568 antibody
      • OTTHUMP00000224066 antibody
      • OTTHUMP00000226924 antibody
      • SMA 1 antibody
      • SMA 2 antibody
      • SMA 3 antibody
      • SMA 4 antibody
      • SMA antibody
      • SMA@ antibody
      • SMA1 antibody
      • SMA2 antibody
      • SMA3 antibody
      • SMA4 antibody
      • SMN antibody
      • SMN_HUMAN antibody
      • SMN1 antibody
      • SMN2 antibody
      • SMNT antibody
      • Survival motor neuron protein antibody
      • Survival of motor neuron 1, telomeric antibody
      • T-BCD541 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      ICC/IF image of ab5831 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      IHC image of ab5831 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5831, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Western blot - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      All lanes : Anti-SMN/Gemin 1 antibody [2B1] (ab5831) at 1 µg/ml

      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 35 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      Gemin 1contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
    • Immunoprecipitation - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Immunoprecipitation - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Gemin 1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Gemin 1 (ab5831) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min,Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5831.
      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
      Band: 32kDa: Gemin 1
    • Flow Cytometry - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Flow Cytometry - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Overlay histogram showing HepG2 cells stained with ab5831 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5831, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)Image courtesy of an anonymous Abreview.
      ab5831 staining Gemin 1 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 0.2% fish scale gelatin for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/300 for 20 minutes at 25°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. Counterstained with DAPI (blue).
      Gemin 1 is clearly visible in the cytoplasm and also as small dots in the nucleus (cajal bodies).
    • Western blot - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)
      Western blot - Anti-SMN/Gemin 1 antibody [2B1] (ab5831)This image is courtesy of an abreview submitted by Samantha Jeschonek.
      Anti-SMN/Gemin 1 antibody [2B1] (ab5831) at 1/100 dilution + Xenopus laevis st II-III oocytes whole cell lysate at 20 µg

      Secondary
      Goat Anti-Mouse IgG Fc (HRP) (ab97265) at 1/25000 dilution

      Performed under reducing conditions.

      Predicted band size: 35 kDa
      Observed band size: 31 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds

      See Abreview

    文献 (16)

    发表研究结果有使用 ab5831？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab5831 被引用在 16 文献中.

    • Ni SY  et al. Puerarin Alleviates Lipopolysaccharide-Induced Myocardial Fibrosis by Inhibiting PARP-1 to Prevent HMGB1-Mediated TLR4-NF-?B Signaling Pathway. Cardiovasc Toxicol 20:482-491 (2020). PubMed: 32236896
    • Zhu L  et al. Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA-20a-mediated activation of the PTEN/PI3K/AKT signaling pathway. Int J Mol Med 46:2035-2046 (2020). PubMed: 33125088
    • Zhang Y  et al. Silencing Nogo-B receptor inhibits penile corpus cavernosum vascular smooth muscle cell apoptosis of rats with diabetic erectile dysfunction by down-regulating ICAM-1. PLoS One 14:e0220715 (2019). PubMed: 31442237
    • Che X  et al. Long noncoding RNA MEG3 suppresses podocyte injury in diabetic nephropathy by inactivating Wnt/ß-catenin signaling. PeerJ 7:e8016 (2019). PubMed: 31799068
    • Huang L  et al. Antibacterial poly (ethylene glycol) diacrylate/chitosan hydrogels enhance mechanical adhesiveness and promote skin regeneration. Carbohydr Polym 225:115110 (2019). PubMed: 31521272
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-Gemin 1 antibody [2B1]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - whole cell (st II-III oocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20% precast)
    Loading amount
    20 cells
    Specification
    st II-III oocytes
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More

    Ms. Samantha Jeschonek

    Verified customer

    提交于 Feb 03 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com