Anti-SMNDC1抗体(ab227952)
Key features and details
- Rabbit polyclonal to SMNDC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SMNDC1抗体
参阅全部 SMNDC1 一抗 -
描述
兔多克隆抗体to SMNDC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig, Rhesus monkey -
免疫原
Recombinant fragment within Human SMNDC1 (internal sequence). The exact sequence is proprietary.
Database link: O75940 -
阳性对照
- IHC-P: Human lung cancer tissue; C2C12 and SNU1 xenograft tissues. WB: IMR32 whole cell lysate. ICC/IF: MCF7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227952于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Necessary for spliceosome assembly. Overexpression causes apoptosis. -
组织特异性
Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas. -
序列相似性
Belongs to the SMN family.
Contains 1 Tudor domain. -
细胞定位
Nucleus speckle. Nucleus > Cajal body. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 520500 Cow
- Entrez Gene: 10285 Human
- Entrez Gene: 76479 Mouse
- Entrez Gene: 287768 Rat
- Omim: 603519 Human
- SwissProt: Q3T045 Cow
- SwissProt: O75940 Human
- SwissProt: Q8BGT7 Mouse
see all -
别名
- 30 kDa splicing factor SMNrp antibody
- MGC106917 antibody
- MGC112663 antibody
see all
图片
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Anti-SMNDC1 antibody (ab227952) at 1/1000 dilution + IMR32 (human neuroblast cell line) whole cell lysate at 30 µg
Predicted band size: 27 kDa12% SDS-PAGE gel.
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Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for SMNDC1 (green) using ab227952 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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Paraffin-embedded human lung cancer tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded C2C12 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded SNU1 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227952 尚未被引用在任何文献中。