概述

  • 产品名称

    Anti-SMC1A (phospho S966)抗体
    参阅全部 SMC1A 一抗
  • 描述

    兔多克隆抗体to SMC1A (phospho S966)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, IP, WB, ICC/IF, ICCmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to SMC1A conjugated to keyhole limpet haemocyanin.

  • 阳性对照

    • HeLa cells treated with 10 Gy IR for 1hr. IHC-P: FFPE human spleen tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab1276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-6 µg/mg of lysate.
WB 1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

靶标

  • 功能

    Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
  • 疾病相关

    Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
  • 序列相似性

    Belongs to the SMC family. SMC1 subfamily.
  • 结构域

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • 翻译后修饰

    Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
  • 细胞定位

    Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
  • Information by UniProt
  • 数据库链接

  • 别名

    • Chromosome segregation protein SmcB antibody
    • DXS423E antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • Sb1.8 antibody
    • Segregation of mitotic chromosomes 1 antibody
    • SMC protein 1A antibody
    • SMC-1-alpha antibody
    • SMC-1A antibody
    • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
    • SMC1 antibody
    • SMC1 structural maintenance of chromosomes 1 like 1 antibody
    • SMC1A antibody
    • SMC1A_HUMAN antibody
    • SMC1alpha antibody
    • SMC1L1 antibody
    • SMCB antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes protein 1A antibody
    see all

图片

  • ab1276 at a 1:1000 dilution staining phospho SMC1A (Ser 966) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract by Western Blot (ECL). ab1276 at a 1:1000 dilution staining phospho SMC1A (Ser 966) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract by Western Blot (ECL).

  • Immunocytochemistry/Immunofluorescence analysis of neocarzinostatin treated asynchronous HeLa cells labelling SMC1A (phospho S966) with ab1276 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

  • IHC image of SMC1A (phospho S966)  staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1276, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1A (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).

文献

This product has been referenced in:

  • Zhang Y  et al. Phosphorylation of SMC1A promotes hepatocellular carcinoma cell proliferation and migration. Int J Biol Sci 14:1081-1089 (2018). Read more (PubMed: 29988990) »
  • Yang T  et al. Quercetin-3-O-ß-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats. Mol Med Rep 18:2599-2612 (2018). Read more (PubMed: 30015887) »
See all 15 Publications for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your enquiry. I can confirm that the immunogen used to generate ab1276 SMC1 (phospho S966) antibody represents a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2. The sequence NP_006297.2 represents SMC 1A. An alignment of SMC1A and SMC1B indicates that the two isoforms do not bear similar epitopes in this region; therefore I would suggest that ab1276 recognizes S966 in the SMC1A protein. I hope this would be helpful to you. Should you have any further quesitons, please do not hesitate to contact us.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Treatment
X radiation (10g)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

提交于 Mar 28 2007

Application
Immunocytochemistry
Sample
Human Cell (HeLa and SK-N-SH)
Specification
HeLa and SK-N-SH
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

提交于 Oct 12 2005

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