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Cell Biology Cell Cycle Cell Division Chromatid Cohesion

Anti-SMC1A (phospho S957)抗体(ab1275)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (4)

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Western blot - Anti-SMC1A (phospho S957) antibody (ab1275)

    Key features and details

    • Rabbit polyclonal to SMC1A (phospho S957)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-SMC1A (phospho S957)抗体
      参阅全部 SMC1A 一抗
    • 描述

      兔多克隆抗体to SMC1A (phospho S957)
    • 宿主

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • 免疫原

      Synthetic peptide corresponding to SMC1A conjugated to keyhole limpet haemocyanin.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for SMC1A pSer957 were affinity purified using the phosphopeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280nm of 1.4 equals 1.0 mg of IgG.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Chromatid Cohesion
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Homologous Recomb.
      • Epigenetics and Nuclear Signaling
      • Chromosome Structure
      • Cohesins
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Chromosome Structure
      • Chromatid Cohesion

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1275 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Human
    All applications
    Rabbit
    Guinea pig
    Cow
    Dog
    Pig
    Ferret
    Rhesus monkey
    Orangutan
    Elephant
    应用 Ab评论 说明
    WB
    1/200 - 1/2000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
    说明
    WB
    1/200 - 1/2000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).

    靶标

    • 功能

      Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
    • 疾病相关

      Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
    • 序列相似性

      Belongs to the SMC family. SMC1 subfamily.
    • 结构域

      The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
    • 翻译后修饰

      Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
    • 细胞定位

      Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
    • Target information above from: UniProt accession Q14683 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 282370 Cow
      • Entrez Gene: 8243 Human
      • Omim: 300040 Human
      • SwissProt: O97593 Cow
      • SwissProt: Q14683 Human
      • Unigene: 211602 Human
      • 别名

        • Chromosome segregation protein SmcB antibody
        • DXS423E antibody
        • KIAA0178 antibody
        • MGC138332 antibody
        • Sb1.8 antibody
        • Segregation of mitotic chromosomes 1 antibody
        • SMC protein 1A antibody
        • SMC-1-alpha antibody
        • SMC-1A antibody
        • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
        • SMC1 antibody
        • SMC1 structural maintenance of chromosomes 1 like 1 antibody
        • SMC1A antibody
        • SMC1A_HUMAN antibody
        • SMC1alpha antibody
        • SMC1L1 antibody
        • SMCB antibody
        • Structural maintenance of chromosomes 1A antibody
        • Structural maintenance of chromosomes protein 1A antibody
        see all

      图片

      • Western blot - Anti-SMC1A (phospho S957) antibody (ab1275)
        Western blot - Anti-SMC1A (phospho S957) antibody (ab1275)

        ab1275 at a 1:1000 dilution staining phospho SMC1A (Ser 957) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract in Western Blot (ECL).  ab1275 at a 1:1000 dilution staining phospho SMC1A (Ser 957) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract in Western Blot (ECL).

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
      • SDS
    • 文献 (4)

      发表研究结果有使用 ab1275?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1275 被引用在 4 文献中.

      • Mehta K  et al. Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators. PLoS Pathog 11:e1004763 (2015). WB, IP ; Human . PubMed: 25875106
      • Horejsí Z  et al. Distinct functional domains of Nbs1 modulate the timing and magnitude of ATM activation after low doses of ionizing radiation. Oncogene 23:3122-7 (2004). PubMed: 15048089
      • Lukas C  et al. Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage. Nat Cell Biol 5:255-60 (2003). PubMed: 12598907
      • Yazdi PT  et al. SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. Genes Dev 16:571-82 (2002). PubMed: 11877377

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-SMC1 (phospho S957) antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      提交于 Jun 06 2006

      Question

      I am interested in anti SDF1-alpha, and anti CXCR4 antibodies with rat reactivity for immunocytochemistry and would greatly appreciate anything you could suggest.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 24 2005

      Answer

      Thank you for your enquiry. Unfortunately we do not have SDF1 alpha antisera that has been shown to cross react against rat. Both ab7199 and ab13854 have been shown to cross react against rat CXCR4. However, they it is yet to be shown whether they function by immunocytochemistry. You may wish to try the antibody search engine www.biocompare.com.

      Read More

      Abcam Scientific Support

      回复于 Oct 25 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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