概述

  • 产品名称

    Anti-SMC1A (phospho S957)抗体
    参阅全部 SMC1A 一抗
  • 描述

    兔多克隆抗体to SMC1A (phospho S957)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IP, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rabbit, Guinea pig, Cow, Dog, Pig, Ferret, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to SMC1A conjugated to keyhole limpet haemocyanin.

性能

应用

Our Abpromise guarantee covers the use of ab1275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 2 - 6 µg/ml.
WB 1/200 - 1/2000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).

靶标

  • 功能

    Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
  • 疾病相关

    Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
  • 序列相似性

    Belongs to the SMC family. SMC1 subfamily.
  • 结构域

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • 翻译后修饰

    Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
  • 细胞定位

    Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
  • Information by UniProt
  • 数据库链接

  • 别名

    • Chromosome segregation protein SmcB antibody
    • DXS423E antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • Sb1.8 antibody
    • Segregation of mitotic chromosomes 1 antibody
    • SMC protein 1A antibody
    • SMC-1-alpha antibody
    • SMC-1A antibody
    • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
    • SMC1 antibody
    • SMC1 structural maintenance of chromosomes 1 like 1 antibody
    • SMC1A antibody
    • SMC1A_HUMAN antibody
    • SMC1alpha antibody
    • SMC1L1 antibody
    • SMCB antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes protein 1A antibody
    see all

图片

  • ab1275 at a 1:1000 dilution staining phospho SMC1A (Ser 957) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract in Western Blot (ECL).  ab1275 at a 1:1000 dilution staining phospho SMC1A (Ser 957) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract in Western Blot (ECL).

文献

This product has been referenced in:

  • Mehta K  et al. Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators. PLoS Pathog 11:e1004763 (2015). WB, IP ; Human . Read more (PubMed: 25875106) »
  • Horejsí Z  et al. Distinct functional domains of Nbs1 modulate the timing and magnitude of ATM activation after low doses of ionizing radiation. Oncogene 23:3122-7 (2004). Read more (PubMed: 15048089) »
See all 4 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

提交于 Jun 06 2006

Answer

Thank you for your enquiry. Unfortunately we do not have SDF1 alpha antisera that has been shown to cross react against rat. Both ab7199 and ab13854 have been shown to cross react against rat CXCR4. However, they it is yet to be shown whether they function by immunocytochemistry. You may wish to try the antibody search engine www.biocompare.com.

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