Anti-SmarcAL1抗体(ab4743)

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Western blot - Anti-SmarcAL1 antibody (ab4743)

    Key features and details

    • Rabbit polyclonal to SmarcAL1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-SmarcAL1抗体
      参阅全部 SmarcAL1 一抗

    • 描述

      兔多克隆抗体to SmarcAL1

    • 宿主

      Rabbit

    • 特异性

      This antibody detects a band at 105kD which corresponds to SmarcAL1. It also detects several lower weight bands of unknown identity.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide:

      SNADQRPHDSHSFQAKGC

      , corresponding to amino acids 80-96 of Human SmarcAL1.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab4743 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

    靶标

    • 功能

      ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.

    • 组织特异性

      Ubiquitously expressed, with high levels in testis.

    • 疾病相关

      Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.

    • 序列相似性

      Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
      Contains 2 HARP domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • HARP antibody
        • HepA Related Protein antibody
        • HepA-related protein antibody
        • hHARP antibody
        • SIOD antibody
        • SMAL1_HUMAN antibody
        • SMARCA like Protein 1 antibody
        • smarcal1 antibody
        • Sucrose nonfermenting protein 2 like 1 antibody
        • Sucrose nonfermenting protein 2-like 1 antibody
        • SWI/SNF Related antibody
        • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A like protein 1 antibody
        • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 antibody
        see all

      图片

      • Western blot - Anti-SmarcAL1 antibody (ab4743)
        Western blot - Anti-SmarcAL1 antibody (ab4743)

        Western blot using ab4743 at 1/500.

        Lane 1: Hela nuclear
        Lane 2: Hela
        Lane 3: A431
        Lane 4: Jurkat
        Lane 5: 293

        SmarcAL1 is the band seen between the 97 and 116kD markers.

        Secondary antibody: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
        Exposure time: 1 minute
        Cell lysates: 5µg per lane.

        Western blot using ab4743 at 1/500.

        Lane 1: Hela nuclear
        Lane 2: Hela
        Lane 3: A431
        Lane 4: Jurkat
        Lane 5: 293

        SmarcAL1 is the band seen between the 97 and 116kD markers.

        Secondary antibody: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
        Exposure time: 1 minute
        Cell lysates: 5µg per lane.

      文献 (0)

      发表研究结果有使用 ab4743？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab4743 尚未被引用在任何文献中。

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