Application
Western blot
Sample
Human Cell lysate - whole cell (Human prostate cancer cell)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
30 µg
Treatment
TGF beta 5ng/ml
Specification
Human prostate cancer cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Hela cell with no treatment
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 54 kDa
Positive control
Hela cells with TGF beta treatment
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 27 2015