Anti-Smad2 + Smad3抗体(ab217553)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Smad2 + Smad3抗体
参阅全部 Smad2 + Smad3 一抗 -
描述
兔多克隆抗体to Smad2 + Smad3 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Smad2 + Smad3 aa 30-80 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q15796 -
阳性对照
- Flow Cyt: HUVEC cells ICC/IF: HeLa cells WB: Mouse and Rat cerebrum, testis and kidney lysates, mouse heart, mouse skin, HUVEC cells, A549 cells, HeLa cells, HT1080 cells, A431 cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab217553于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/100 - 1/1000.
|
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Flow Cyt |
1/50.
|
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ICC/IF |
1/100.
|
说明 |
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WB
1/100 - 1/1000. |
Flow Cyt
1/50. |
ICC/IF
1/100. |
靶标
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相关性
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. -
细胞定位
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. -
数据库链接
- Entrez Gene: 4087 Human
- Entrez Gene: 4088 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 17127 Mouse
- Entrez Gene: 25631 Rat
- Entrez Gene: 29357 Rat
- Omim: 601366 Human
- Omim: 603109 Human
see all -
别名
- hMAD 2 antibody
- hMAD 3 antibody
- hSMAD2 antibody
see all
图片
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All lanes : Anti-Smad2 + Smad3 antibody (ab217553) at 1/1000 dilution
Lane 1 : Mouse cerebrum lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse testis lysate
Lane 4 : Mouse Skin lysate
Lane 5 : Mouse Kidney lysate
Lane 6 : Rat cerebrum lysate
Lane 7 : Rat Testis lysate
Lane 8 : Rat kidney lysate
Lane 9 : HUVEC cell lysate
Lane 10 : A459 cell lysate
Lane 11 : HeLa cell lysate
Lane 12 : HT1080 cell lysate
Lane 13 : A431 cell lysate -
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Smad3) polyclonal Antibody, Unconjugated (ab217553) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
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HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad2/3 Polyclonal Antibody(ab217553) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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All lanes : Anti-Smad2 + Smad3 antibody (ab217553) at 1/200 dilution
Lane 1 : mouse brain lysates
Lane 2 : mouse kidney lysates
Secondary
All lanes : HRP-conjugated secondary antibody at 1/3000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?12% SDS-PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab217553 被引用在 6 文献中.
- Kang Y et al. MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children. Iran J Public Health 50:1973-1982 (2021). PubMed: 35223564
- Li L et al. The dipeptidyl peptidase-4 inhibitor sitagliptin ameliorates renal injury in type 1 diabetic mice via inhibiting the TGF-ß/Smad signal pathway. Pharmazie 74:239-242 (2019). PubMed: 30940309
- Liu Y et al. Quercetin suppresses glomerulosclerosis and TGF-ß signaling in a rat model. Mol Med Rep 19:4589-4596 (2019). PubMed: 30942399
- Adhikary S et al. FGF-2 targets sclerostin in bone and myostatin in skeletal muscle to mitigate the deleterious effects of glucocorticoid on musculoskeletal degradation. Life Sci 229:261-276 (2019). PubMed: 31082400
- Li L et al. Recombinant truncated TGF-ß receptor II attenuates carbon tetrachloride-induced epithelial-mesenchymal transition and liver fibrosis in rats. Mol Med Rep 17:315-321 (2018). PubMed: 29115426
- Huang T et al. STK39, overexpressed in osteosarcoma, regulates osteosarcoma cell invasion and proliferation. Oncol Lett 14:4599-4604 (2017). WB ; Human . PubMed: 28943960