Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (st.10.5 embryo)
Permeabilization
No
Specification
st.10.5 embryo
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: T-PBS
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG antibodies
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
No signals were detected in Xenopus embryo under the following conditions.
Fixing solution: 2% PFA, 4%PFA, Bounin's fixing fluid, methanol-formalin, 4%PFA-20% methanol
Antigen retrieval: no treatment, 10 mM citrate buffer (pH 6), 1mM Tris-EDTA buffer (pH 9), HCl buffer
Dilution of primary antibody: 1/100, 1/200, 1/500, 1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 20 2016