重组Anti-Smac/Diablo抗体[Y12] (ab32023)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y12] to Smac/Diablo
- Suitable for: WB, IHC-P, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Smac/Diablo抗体[Y12]
参阅全部 Smac/Diablo 一抗 -
描述
兔单克隆抗体[Y12] to Smac/Diablo -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IP, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Smac/ Diablo (C terminal). The exact sequence is proprietary.
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表位
ab32023 reacts with an epitope located in the C terminal region of Smac / Diablo. -
阳性对照
- WB: Jurkat whole cell lysate (ab7899). IHC-P: Human skin cancer tissue. ICC/IF: HeLa cells. IP: Jurkat.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y12 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32023于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa).
|
IHC-P |
1/10. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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IP | (1) |
1/10 - 1/80.
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ICC/IF |
1/500.
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说明 |
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WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/10. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/80. |
ICC/IF
1/500. |
靶标
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功能
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
组织特异性
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
结构域
The mature N-terminus mediates interaction with XIAP. -
细胞定位
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- Unigene: 169611 Human
- Unigene: 46716 Mouse
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别名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
图片
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Anti-Smac/Diablo antibody [Y12] (ab32023) at 1/1000 dilution + Jurkat cell lysate
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Smac / Diablo with ab32023 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
ab32023, at a 1/10 dilution, staining human Smac/Diablo in skin cancer tissue by immunohistochemistry, paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of IMR32 cells, staining Smac / Diablo with ab32023. Cells were fixed with 4% paraformaldehyde before incubating with primary antibody. An Alexa Fluor® conjugated anti-rabbit IgG was used as the secondary antibody.
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Smac/Diablo was immunoprecipitated from 0.35 mg Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 μg with ab32023 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 μg
Lane 2: ab32023 IP in Jurkat whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab32023 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab32023 被引用在 22 文献中.
- García-Gutiérrez L et al. Interaction of LATS1 with SMAC links the MST2/Hippo pathway with apoptosis in an IAP-dependent manner. Cell Death Dis 13:692 (2022). PubMed: 35941108
- McKenna S et al. BAX and SMAC regulate bistable properties of the apoptotic caspase system. Sci Rep 11:3272 (2021). PubMed: 33558564
- Xiang RP et al. Effects of Different Degrees of Carotid Artery Stenosis on the Expression of XIAP and Smac in the Ischemic Penumbra of Rats with Cerebral Ischemia-Reperfusion. J Stroke Cerebrovasc Dis 30:105516 (2021). PubMed: 33310074
- Zhou H et al. Upconversion NIR-II fluorophores for mitochondria-targeted cancer imaging and photothermal therapy. Nat Commun 11:6183 (2020). PubMed: 33273452
- Xian H & Liou YC Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy. Autophagy 15:2107-2125 (2019). PubMed: 30894073