重组Anti-Smac/Diablo抗体[EPR10934] (ab174284)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10934] to Smac/Diablo
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Smac/Diablo抗体[EPR10934]
参阅全部 Smac/Diablo 一抗 -
描述
兔单克隆抗体[EPR10934] to Smac/Diablo -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment within Human Smac/ Diablo. The exact sequence is proprietary.
Database link: Q9NR28 -
阳性对照
- HeLa, MCF7, Jurkat, 293T and Human testis lysates; Human heart and testis tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR10934 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174284于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
|
说明 |
---|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Predicted molecular weight: 27 kDa. |
靶标
-
功能
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
组织特异性
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
结构域
The mature N-terminus mediates interaction with XIAP. -
细胞定位
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
-
数据库链接
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- Unigene: 169611 Human
- Unigene: 46716 Mouse
-
别名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
图片
-
Immunohistochemical analysis of paraffinn embedded Human testis tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffinn embedded Human heart tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-Smac/Diablo antibody [EPR10934] (ab174284) at 1/1000 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : Jurkat cell line lysate
Lane 4 : 293T cell line lysate
Lane 5 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab174284 尚未被引用在任何文献中。