Anti-SLK抗体(ab65113)
Key features and details
- Rabbit polyclonal to SLK
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SLK抗体
参阅全部 SLK 一抗 -
描述
兔多克隆抗体to SLK -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65113于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/500 - 1/1000.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
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IHC-P |
1/50 - 1/100.
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说明 |
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ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa). |
IHC-P
1/50 - 1/100. |
靶标
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相关性
SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 9748 Human
- SwissProt: Q9H2G2 Human
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别名
- CTCL tumor antigen se20 9 antibody
- LOSK antibody
- se20 9 antibody
see all
图片
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This image shows human skeletal muscle tissue stained with ab65113 at 1/50 - 1/100 dilution; with and without the immunising peptide.
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All lanes : Anti-SLK antibody (ab65113) at 1/500 dilution
Lane 1 : extracts from COLO205 cells
Lane 2 : extracts from COLO205 cells with immunising peptide
Predicted band size: 143 kDa
Observed band size: 143 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug. -
Immunofluorescence analysis of HepG2 cells, using ab65113 antibody at 1/500-1/1000 dilution
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab65113 被引用在 2 文献中.
- Tranchevent LC et al. Identification of protein features encoded by alternative exons using Exon Ontology. Genome Res 27:1087-1097 (2017). ICC/IF ; Human . PubMed: 28420690
- Müller T et al. ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients. Oncogene : (2010). WB ; Human . PubMed: 20154719