重组Anti-Slit2抗体[EPR2771] (ab134166)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2771] to Slit2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Slit2抗体[EPR2771]
参阅全部 Slit2 一抗 -
描述
兔单克隆抗体[EPR2771] to Slit2 -
宿主
Rabbit -
特异性
Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Slit2 aa 1450-1550. The exact sequence is proprietary.
Database link: O94813 -
阳性对照
- WB: HEK-293, Untreated PC-3 and treated with 300ng/ml Brefeldin A (BFA) for 24 hours whole cell lysates. IHC: Human fetal kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2771 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134166于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 169 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 169 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. -
组织特异性
Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
结构域
The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro. -
细胞定位
Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated. - Information by UniProt
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数据库链接
- Entrez Gene: 9353 Human
- Entrez Gene: 20563 Mouse
- Entrez Gene: 360272 Rat
- Omim: 603746 Human
- SwissProt: O94813 Human
- SwissProt: Q9R1B9 Mouse
- SwissProt: Q9WVC1 Rat
- Unigene: 29802 Human
see all -
别名
- Drad 1 antibody
- E030015M03Rik antibody
- E130320P19Rik antibody
see all
图片
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All lanes : Anti-Slit2 antibody [EPR2771] (ab134166) at 1/1000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : T-47(Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : Untreated PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : PC-3 (Human prostate adenocarcinoma epithelial cell) treated with 300ng/ml Brefeldin A (BFA) for 24 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 169 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
The expression level of Slit2 in T-47D and A549 are consistent with describes in PMID: 24287947 and PMID: 12384551.
Negative control: T-47D and A549 (PMID: 24287947 and PMID: 12384551).
Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.
ab181602 was used as a loading control.
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Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab134166 被引用在 19 文献中.
- Feng XF et al. Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats. Front Pharmacol 13:851746 (2022). PubMed: 35559236
- Kong M et al. Expression of the axon guidance factor Slit2 and its receptor Robo1 in patients with Hirschsprung disease: An observational study. Medicine (Baltimore) 100:e26981 (2021). PubMed: 34414975
- Huo Z et al. Rho-kinase inhibition by Fasudil promotes tumor maturation and apoptosis in small-cell lung cancer. Am J Transl Res 12:4354-4370 (2020). PubMed: 32913511
- Tavora B et al. Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis. Nature 586:299-304 (2020). PubMed: 32999457
- Zhan Y et al. An MRI Study of Neurovascular Restorative After Combination Treatment With Xiaoshuan Enteric-Coated Capsule and Enriched Environment in Rats After Stroke. Front Neurosci 13:701 (2019). PubMed: 31354412