重组Anti-Slit2抗体[EPR23272-227] (ab246503)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23272-227] to Slit2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Slit2抗体[EPR23272-227]
参阅全部 Slit2 一抗 -
描述
兔单克隆抗体[EPR23272-227] to Slit2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: rat brain tissue, PC-3, T-47, mIMCD3, Mouse E14.5 brain tissue, 293T.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23272-227 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab246503于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 169 kDa.
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说明 |
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WB
1/1000. Predicted molecular weight: 169 kDa. |
靶标
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功能
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. -
组织特异性
Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
结构域
The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro. -
细胞定位
Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated. - Information by UniProt
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数据库链接
- Entrez Gene: 9353 Human
- Entrez Gene: 20563 Mouse
- Entrez Gene: 360272 Rat
- Omim: 603746 Human
- SwissProt: O94813 Human
- SwissProt: Q9R1B9 Mouse
- SwissProt: Q9WVC1 Rat
- Unigene: 29802 Human
see all -
别名
- Drad 1 antibody
- E030015M03Rik antibody
- E130320P19Rik antibody
see all
图片
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Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution + Rat brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated)
Predicted band size: 169 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).
The molecular weight observed is consistent with what has been described in the literature (PMID: 11404413).
Exposure time: 3 minutes.
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All lanes : Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : T-47D(Human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg
Lane 3 : mIMCD3 (mouse inner medlary collecting duct epithelial cell), whole cell lysate at 20 µl
Lane 4 : Mouse E14.5 brain tissue lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 169 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
A~25 kDa degraded band is observed. Freshly made lysates can decrease the degradation.
The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).
The molecular weight observed is consistent with what has been described in the literature (PMID: 11404413).
Negative control: T-47D (PMID:17268810)
Exposure time:
Lanes 1-2: 136 seconds;
Lanes 3-4: 26 seconds.
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All lanes : Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution
All lanes : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 169 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Fresh lysate was used in lane 1.
Band detected around 25KDa in lane 2 is caused by degradation as it disappeared in fresh lysate.
Exposure time: 37 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab246503 尚未被引用在任何文献中。