Anti-SLC16A3/MCT 4抗体(ab234728)
Key features and details
- Rabbit polyclonal to SLC16A3/MCT 4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SLC16A3/MCT 4抗体
参阅全部 SLC16A3/MCT 4 一抗 -
描述
兔多克隆抗体to SLC16A3/MCT 4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human SLC16A3/MCT 4 aa 400 to the C-terminus.
Database link: O15427 -
阳性对照
- WB: HeLa and MCF-7. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab234728于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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ICC/IF |
1/50 - 1/200.
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说明 |
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WB
1/500 - 1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
ICC/IF
1/50 - 1/200. |
靶标
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功能
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. -
组织特异性
Highly expressed in skeletal muscle. -
序列相似性
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9123 Human
- Omim: 603877 Human
- SwissProt: O15427 Human
- Unigene: 500761 Human
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形式
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains. -
别名
- MCT 4 antibody
- MCT3 antibody
- MCT4 antibody
see all
图片
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All lanes : Anti-SLC16A3/MCT 4 antibody (ab234728) at 1/2500 dilution
Lane 1 : MCF-7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/5000 dilution
Predicted band size: 49 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLC16A3/MCT 4 using ab234728 at a dilution of 1/100 in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab234728 被引用在 3 文献中.
- Deguchi H et al. Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy. Sci Rep 12:6263 (2022). PubMed: 35428816
- Shrestha P et al. Monocarboxylate Transporters: Role and Regulation in Corneal Diabetes. Anal Cell Pathol (Amst) 2022:6718566 (2022). PubMed: 36340268
- Mou Z et al. Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma. Front Genet 11:566918 (2020). PubMed: 33424916