重组Anti-SIRT2抗体[EPR20411-105] (ab211033)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20411-105] to SIRT2
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SIRT2抗体[EPR20411-105]
参阅全部 SIRT2 一抗 -
描述
兔单克隆抗体[EPR20411-105] to SIRT2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IHC-Fr, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal brain and brain cortex lysates; Mouse brain and cerebral cortex lysates; Rat brain lysate; MCF7 whole cell lysate. IHC-P: Human, mouse and rat cerebrum tissues. IHC-Fr: Mouse striatum tissue. IP: Human fetal brain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20411-105 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab211033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/2000. Detects a band of approximately 36, 43 kDa (predicted molecular weight: 43 kDa).
|
IHC-P |
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
Anitgen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP |
1/30.
|
说明 |
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WB
1/2000. Detects a band of approximately 36, 43 kDa (predicted molecular weight: 43 kDa). |
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. Anitgen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
IP
1/30. |
靶标
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功能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
组织特异性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
序列相似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
发展阶段
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻译后修饰
Phosphorylated at the G2/M transition of the cell cycle. -
细胞定位
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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数据库链接
- Entrez Gene: 22933 Human
- Entrez Gene: 64383 Mouse
- Entrez Gene: 361532 Rat
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- SwissProt: Q8VDQ8 Mouse
- SwissProt: Q5RJQ4 Rat
- Unigene: 466693 Human
see all -
别名
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
图片
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Lane 1 : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/5000 dilution
Lane 2 : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human brain cortex lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 36,43 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 8 seconds; Lane 2: 1 second.
SIRT2 exists in two main isoforms, SIRT2.1 (43 kDa) and SIRT2.2 (39 kDa). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:21791548).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse striatum tissue labeling SIRT2 with ab211033 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse stratium is observed [PMID: 17447894]. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of human cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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SIRT2 was immunoprecipitated from 0.35 mg of Human fetal brain lysate with ab211033 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211033 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human fetal brain lysate, 10 μg (Input).
Lane 2: ab211033 IP in Human fetal brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211033 in Human fetal brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse cerebral cortex lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 minutes; Lane 2/3: 5 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:21791548).
-
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of mouse cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of rat cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (12)
ab211033 被引用在 12 文献中.
- Li J et al. Oxygen-carrying sequential preservation mitigates liver grafts ischemia-reperfusion injury. iScience 26:105858 (2023). PubMed: 36636350
- Siqueira E et al. Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome. Mol Ther Nucleic Acids 27:621-644 (2022). PubMed: 35036070
- Jablonska B et al. Sirt2 promotes white matter oligodendrogenesis during development and in models of neonatal hypoxia. Nat Commun 13:4771 (2022). PubMed: 35970992
- Cao Z et al. FHL1 mediates HOXA10 deacetylation via SIRT2 to enhance blastocyst-epithelial adhesion. Cell Death Discov 8:461 (2022). PubMed: 36418297
- Yi F et al. The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis. Sci Adv 7:N/A (2021). PubMed: 33627431