重组Anti-SIRT2抗体[EP1668Y] (ab51023)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1668Y] to SIRT2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SIRT2抗体[EP1668Y]
参阅全部 SIRT2 一抗 -
描述
兔单克隆抗体[EP1668Y] to SIRT2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: SKBR-3 cell lysate Flow Cyt (intra): HeLa cells IP: HeLa cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP1668Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51023于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (3) |
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
|
IP | (1) |
1/50.
|
说明 |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa). |
IP
1/50. |
靶标
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功能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
组织特异性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
序列相似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
发展阶段
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻译后修饰
Phosphorylated at the G2/M transition of the cell cycle. -
细胞定位
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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数据库链接
- Entrez Gene: 22933 Human
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- Unigene: 466693 Human
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别名
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
图片
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Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Purified ab51023 at 1/50 dilution (2µg) immunoprecipitating SIRT2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab51023 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51023 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36-43 kDa -
Intracellular Flow Cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red) (ab51023) or a rabbit IgG (negative) (green).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab51023 被引用在 13 文献中.
- Zhang X et al. Sirtuin 2 Alleviates Chronic Neuropathic Pain by Suppressing Ferroptosis in Rats. Front Pharmacol 13:827016 (2022). PubMed: 35401208
- Zhao Y et al. SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy. Aging Cell 20:e13391 (2021). PubMed: 34053152
- Ni Y et al. Silent information regulator 2 promotes clear cell renal cell carcinoma progression through deacetylation and small ubiquitin-related modifier 1 modification of glucose 6-phosphate dehydrogenase. Cancer Sci 112:4075-4086 (2021). PubMed: 34310804
- Cha Y et al. SIRT2 regulates mitochondrial dynamics and reprogramming via MEK1-ERK-DRP1 and AKT1-DRP1 axes. Cell Rep 37:110155 (2021). PubMed: 34965411
- Martínez-Gutiérrez A et al. Shikimic acid protects skin cells from UV-induced senescence through activation of the NAD+-dependent deacetylase SIRT1. Aging (Albany NY) 13:12308-12333 (2021). PubMed: 33901008