Anti-SIRP alpha抗体(ab232765)
Key features and details
- Rabbit polyclonal to SIRP alpha
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-SIRP alpha抗体
参阅全部 SIRP alpha 一抗 -
描述
兔多克隆抗体to SIRP alpha -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Pig -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human SIRP alpha aa 100-350. Expressed in E.coli. N-terminal tags.
Database link: P78324-1 -
阳性对照
- WB: Recombinant human SIRP alpha protein; HEK-293T cell lysate; Pig brain lysate. IHC-P: Human cerebrum, liver, breast cancer and stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232765于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
组织特异性
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
序列相似性
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 140885 Human
- Entrez Gene: 494566 Pig
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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别名
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
图片
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-SIRP alpha antibody (ab232765) at 2 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
Formalin-fixed, paraffin-embedded human liver tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-SIRP alpha antibody (ab232765) at 2 µg + Recombinant human SIRP alpha protein
Predicted band size: 55 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab232765 尚未被引用在任何文献中。