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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-SIL1抗体(ab5639)

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Western blot - Anti-SIL1 antibody (ab5639)

    Key features and details

    • Goat polyclonal to SIL1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-SIL1抗体
      参阅全部 SIL1 一抗
    • 描述

      山羊多克隆抗体to SIL1
    • 宿主

      Goat
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide:

      ELLGSVNSLLKELR

      , corresponding to C terminal amino acids 448-461 of Human SIL1.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明


      SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Primary antibody说明

      SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Heat Shock Proteins
      • Signal Transduction
      • Protein Trafficking
      • ER Proteins
      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Other Chaperones

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human SIL1 protein (ab128448)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5639于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 53 kDa).

    A 1 hour primary incubation is recommended for this product.

    说明
    WB
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 53 kDa).

    A 1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
    • 组织特异性

      Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.
    • 疾病相关

      Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS.
    • 序列相似性

      Belongs to the SIL1 family.
    • 发展阶段

      Expressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain.
    • 翻译后修饰

      N-glycosylated.
    • 细胞定位

      Endoplasmic reticulum lumen.
    • Target information above from: UniProt accession Q9H173 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 64374 Human
      • Omim: 608005 Human
      • SwissProt: Q9H173 Human
      • Unigene: 483521 Human
      • 别名

        • BAP antibody
        • BiP associated protein antibody
        • BiP-associated protein antibody
        • Endoplasmic reticulum chaperone SIL 1 antibody
        • Endoplasmic reticulum chaperone SIL1 antibody
        • MSS antibody
        • Nucleotide exchange factor SIL 1 antibody
        • Nucleotide exchange factor SIL1 antibody
        • SIL 1 antibody
        • sil1 antibody
        • SIL1 homolog antibody
        • SIL1 homolog endoplasmic reticulum chaperone (S. cerevisiae) antibody
        • SIL1 homolog endoplasmic reticulum chaperone antibody
        • SIL1_HUMAN antibody
        • UGL 5 antibody
        • ULG5 antibody
        see all

      图片

      • Western blot - Anti-SIL1 antibody (ab5639)
        Western blot - Anti-SIL1 antibody (ab5639)

        ab5639 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.



        ab5639 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (6)

      发表研究结果有使用 ab5639?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab5639 被引用在 6 文献中.

      • Gatz C  et al. Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sjögren Syndrome. Front Neurol 10:562 (2019). PubMed: 31258504
      • Xu H  et al. SIL1 functions as an oncogene in glioma by AKT/mTOR signaling pathway. Onco Targets Ther 11:3775-3783 (2018). PubMed: 29997438
      • Filézac de L'Etang A  et al. Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS. Nat Neurosci 18:227-38 (2015). IHC-FoFr, IHC-P ; Mouse, Human . PubMed: 25559081
      • Anttonen AK  et al. Novel SIL1 mutations and exclusion of functional candidate genes in Marinesco-Sjögren syndrome. Eur J Hum Genet 16:961-9 (2008). ICC/IF ; Mouse . PubMed: 18285827
      • Liu Y  et al. The orphan receptor tyrosine kinase Ror2 promotes osteoblast differentiation and enhances ex vivo bone formation. Mol Endocrinol 21:376-87 (2007). PubMed: 17095577
      • Anttonen AK  et al. The gene disrupted in Marinesco-Sjögren syndrome encodes SIL1, an HSPA5 cochaperone. Nat Genet 37:1309-11 (2005). IHC-Fr ; Human . PubMed: 16282978

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