Application
FRET
The TR-FRET signal for the labeled-ab6847 + a-Myc-cryptate show a ~44 fold increase compare to the negative (a-Myc-cryptate alone) showing a strong cross reaction between, the ab6847 and the mouse mAb.
Therefore ab6847 cannot be used in presence of mouse mAb due to cross reactivity.
NB. No information on the datasheet which would suggest cross-reactivity , the purification " immunoaffinity chromatography using Rat IgG coupled to agarose beads" let me think that the cross-reactivity to mouse would be low, that's not the case.
Therefore ab6847 cannot be used in presence of mouse mAb due to cross reactivity.
NB. No information on the datasheet which would suggest cross-reactivity , the purification " immunoaffinity chromatography using Rat IgG coupled to agarose beads" let me think that the cross-reactivity to mouse would be low, that's not the case.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 21 2022