羊抗小鼠IgG H&L (FITC) (ab6804)

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Key features and details

  • Sheep Anti-Mouse IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Sheep
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

Conjugates logo Related conjugates and formulations

Biotin HRP Texas Red ® TRITC Unconjugated

概述

  • 产品名称

    羊抗小鼠IgG H&L (FITC)
    参阅全部 IgG 二抗

  • 宿主

    Sheep

  • 靶标种属

    Mouse

  • 经测试应用

    适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

  • 免疫原

    Mouse IgG whole molecule

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG

  • 共轭说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6804于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
说明
应用说明
suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Question

Hi again,

I’ve now tested the new goat antimouse FITC secondary I received from Sigma and it worked very well with the vinculin primary and without any trouble. I still don’t know why the secondaries from abcam never worked, but it’s clear that the problem was with them. Could there have been some sort of issue with storage and shipping? How do I go about requesting a refund?

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Answer

Thank you for getting back to me and sharing your results.

I am very pleased to hear that the new secondary antibody has worked well. This does indeed point to there having been a problem with the secondary antibody that you purchased from us as well as the replacement I sent you. I am not sure of the reason for this but I will be looking into it in more detail. I am very sorry for the inconvenience caused by these problems.

I can arrange for a refund directly for you of the ab6804 if this is what you would like. This will be refunded directly to the account which paid for the original order.Alternatively, I can arrange for any antibody of your choice to be sent instead or raise a credit note which can be used for future purchases.

Please let me know how you would like to proceed and I will arrange it for you.

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Question

Hi,

Answers to the questionnaire attached.

Thanks again.

I'd appreciate any advice.

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Answer

Thank you for getting back to me. I am sorry for my late reply.

I am really not sure where these experiments may be going wrong, with so many different conditions tried I really cannot explain why no staining has been observed in every case.I think I would try to find out a few fundamental things to try and rule outwhat may be contributing to the problems:

1. Have you performed any other staining with a FITC conjugated secondary antibody which has worked? I would check that both of the secondary antibodies are working as expected by putting a little sample directly on a slide and taking an image.

2. Do you have any anti-mouse IgG which you know have been working well with any other primary antibodies? Perhaps with a different conjugate? Could you try initially just to see if the two primary antibodies are detecting the expected protein by using this alternative secondary antibody?

3. You mentioned that co-staining with Alexafluor has worked well. What primary and secondary antibodywere used for this staining? Which antibodies were applied in what order when the dual staining was performed?

I look forward to receiving your reply.

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Question

Hi,

Thank you for sending the replacement antibody. I’ve tried it and I still don’t get any staining. I just can’t figure out what’s wrong with what I’m doing. I’ve done this identical protocol before (but with different antibodies). I never had to do any sort of antigen retrieval or anything like that. I’ve tried with both primaries again and on 2 types of cells. I’m now going to attempt fixing with a fresh batch of PFA and fixing for a shorter period of time (10 minutes instead of 20). I’d be happy to have any other suggestions.

Many thanks,

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Answer

Thank you for getting back to me.

I am sorry to hear that the new secondary antibody has not made a difference in the results obtained. I would be happy to try to help in understanding why this might be happening.I think it would be useful if you could maybe fill in the questionnaire I have attached to this email. This information will allow me to see if there are any suggestions I would make in altering the protocol used.

I think reducing the fixation time and using fresh PFA is a good start and I'll let you know if I have any further suggestions once I have received the completed questionnaire.

I look forward to receiving your reply.

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Question

Many thanks. Please send the Goat secondary (ab97239). Could you make sure it gets sent right away? I'm out of town next week and I'd like to be able to try it before I leave so I can let my student work with it while I'm gone.

All the best,

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Answer

Thank you for confirming that for me.

I have arranged for the replacement to be sent to you. This is on the order number xxxxx(Purchase order number FOCR xxxxxx). This should be with you tomorrow. Please let me know if you have any problems receiving it.

If you continue to have problems in the staining please do also let me know.

Until then, I wish you all the best with your experiments.

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Question

Hi,

The alternative primary antibody was ab16821 (a mouse monoclonal to alpha v integrin). I ordered this antibody on 16 July, but it only arrived a few days ago.

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Answer

Thank you for confirming that.

I can send you a new secondary antibody to try if you would like. I would suggest the following would be suitable:

Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (FITC) (ab97239)

Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab102457)

If you let me know which one you would like to try (or a different one altogether if you prefer). I can then arrange for it to be sent to you, free of charge.

I look forward to receiving your reply.

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Question

Hi again,

We’ve tried the staining again today with the alpha V integrin primary antibody that we ordered with the same order on 16 July. Again, there has been no staining. I’m guessing this means that the secondary antibody is faulty. Could you recommend an alternative, also conjugated to FITC and ship it to me as soon as possible? This has been holding up my work for weeks. I’d be happy to have one raised in Goat if the choices are more limited for those raised in Sheep.

Many thanks,

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Answer

Thank you for getting back to me with that information.

I am sorry but I am a little confused with what has been tested? Which alternative antibody was tried with the secondary antibody which again did not work? On the orderof the 16th of July I only have the ab18058 and ab6804. Do you know the catalogue number of the antibody used?

I will try to resolve this situation as soon as possible. I am sorry for the inconvenience this problem has been causing you.

I look forward to receiving your reply.

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