Anti-SH3BP2 (phospho S427)抗体(ab2176)
Key features and details
- Rabbit polyclonal to SH3BP2 (phospho S427)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SH3BP2 (phospho S427)抗体
参阅全部 SH3BP2 一抗 -
描述
兔多克隆抗体to SH3BP2 (phospho S427)该产品是 快线抗体，通过亲和纯化得到。对抗原肽有高灵敏度及特异性（ELISA测试）。 Read the terms of use »
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宿主
Rabbit -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SH3BP2 aa 400-500 (phospho S427).
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常规说明
Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity purified chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. -
Primary antibody说明
Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
Reacts in Western blotting with a band at about 60kD, that probably corresponds to SH3BP1 (Not tested for phospho specificity by Western). |
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| ELISA |
Use at an assay dependent concentration.
Tested in peptide ELISA against 0.1 ug of the immunizing peptide. |
| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa. Reacts in Western blotting with a band at about 60kD, that probably corresponds to SH3BP1 (Not tested for phospho specificity by Western). |
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ELISA
Use at an assay dependent concentration. Tested in peptide ELISA against 0.1 ug of the immunizing peptide. |
靶标
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功能
Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism. -
组织特异性
Expressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas. -
疾病相关
Defects in SH3BP2 are the cause of cherubism (CRBM) [MIM:118400]. CRBM is an autosomal dominant inherited syndrome characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling. -
序列相似性
Contains 1 PH domain.
Contains 1 SH2 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 6452 Human
- Omim: 602104 Human
- SwissProt: P78314 Human
- Unigene: 167679 Human
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别名
- 3BP-2 antibody
- 3BP2 antibody
- 3BP2_HUMAN antibody
see all
图片
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Western blot - Anti-SH3BP2 (phospho S427) antibody (ab2176)Western blot analysis is shown using ab2176 to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa that probably corresponds to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab2176 尚未被引用在任何文献中。