Key features and details
- Rabbit polyclonal to SH3BP2 (phospho S427)
- Suitable for: WB, ELISA
- Isotype: IgG
产品名称Anti-SH3BP2 (phospho S427)抗体
参阅全部 SH3BP2 一抗
Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Affinity purified chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
Primary antibody说明Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
|WB||Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
Reacts in Western blotting with a band at about 60kD, that probably corresponds to SH3BP1 (Not tested for phospho specificity by Western).
|ELISA||Use at an assay dependent concentration.
Tested in peptide ELISA against 0.1 ug of the immunizing peptide.
功能Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.
组织特异性Expressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas.
疾病相关Defects in SH3BP2 are the cause of cherubism (CRBM) [MIM:118400]. CRBM is an autosomal dominant inherited syndrome characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.
序列相似性Contains 1 PH domain.
Contains 1 SH2 domain.
- Information by UniProt
- 3BP-2 antibody
- 3BP2 antibody
- 3BP2_HUMAN antibody
Western blot analysis is shown using ab2176 to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa that probably corresponds to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.