Anti-SGLT1抗体(ab14686)
Key features and details
- Rabbit polyclonal to SGLT1
- Suitable for: WB, ELISA, Dot blot, IP
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SGLT1抗体
参阅全部 SGLT1 一抗 -
描述
兔多克隆抗体to SGLT1 -
宿主
Rabbit -
经测试应用
适用于: WB, ELISA, Dot blot, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Rat, Human -
免疫原
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阳性对照
- WB: Rat enterocytes, rat heart, and human skeletal muscle tissues lysates.
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常规说明
A blocking peptide for this product is available as ab190911.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified on antigen sepharose affinity column -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14686于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (6) |
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
|
说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa). |
ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
组织特异性
Expressed mainly in intestine and kidney. -
疾病相关
Congenital glucose/galactose malabsorption -
序列相似性
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
翻译后修饰
N-glycosylation is not necessary for the cotransporter function. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6523 Human
- Entrez Gene: 25552 Rat
- Omim: 182380 Human
- SwissProt: P13866 Human
- SwissProt: P53790 Rat
- Unigene: 1964 Human
- Unigene: 10224 Rat
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别名
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
图片
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All lanes : Anti-SGLT1 antibody (ab14686) at 1/500 dilution
Lane 1 : Rat enterocytes
Lane 2 : Rat heart tissue
Lane 3 : Human skeletal muscle tissue
Predicted band size: 73 kDaTissue extracts were made in Solobuffer with protease inhibitors. The samples were solubilized in SDS-PAGE sample buffer and reduced with 2.5% BME.
Total 40-50ug protein was applied per lane on 10% SDS PAGE gels.
The proteins were transferred to nitrocellulose F and blocked with 5% milk and blotted with 1:500 diluted ab14686.The image was obtained with high sensitivity ECL assay and Image was captured with a CCD cooled camera for 30 sec.
数据表及文件
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SDS download
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Datasheet download
文献 (61)
ab14686 被引用在 61 文献中.
- Korfhage J et al. Canagliflozin Increases Intestinal Adenoma Burden in Female ApcMin/+ Mice. J Gerontol A Biol Sci Med Sci 77:215-220 (2022). PubMed: 34448851
- Hirai H et al. The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay. Mol Ther Methods Clin Dev 24:11-19 (2022). PubMed: 34977268
- Abdulrahman N et al. Empagliflozin inhibits angiotensin II-induced hypertrophy in H9c2 cardiomyoblasts through inhibition of NHE1 expression. Mol Cell Biochem 477:1865-1872 (2022). PubMed: 35334035
- Zapater JL et al. Enterocyte HKDC1 Modulates Intestinal Glucose Absorption in Male Mice Fed a High-fat Diet. Endocrinology 163:N/A (2022). PubMed: 35435980
- Zhang Y et al. Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Paeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis. Pharm Biol 60:1237-1254 (2022). PubMed: 35763552