Anti-SFRP1抗体(ab4193)
Key features and details
- Rabbit polyclonal to SFRP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SFRP1抗体
参阅全部 SFRP1 一抗 -
描述
兔多克隆抗体to SFRP1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
IGPYQSGRFYTKC
, corresponding to amino acids 42 - 53 of Human SFRP1. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB | (1) |
1/230. Predicted molecular weight: 37 kDa.
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/230. Predicted molecular weight: 37 kDa. |
靶标
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功能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
组织特异性
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
序列相似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
结构域
The FZ domain is involved in binding with Wnt ligands. -
细胞定位
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
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数据库链接
- Entrez Gene: 6422 Human
- Omim: 604156 Human
- SwissProt: Q8N474 Human
- Unigene: 213424 Human
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别名
- Frizzled related protein 1 antibody
- FRP 1 antibody
- FRP antibody
see all
图片
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Western blot - Anti-SFRP1 antibody (ab4193)This image is courtesy of Becky Mercer, University of Columbia
Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.
Expected band size: 37kD.
Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.
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ICC/IF image of ab4193 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4193, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab4193 被引用在 40 文献中.
- Wu T et al. The clinical treatment and outcome of cerebellopontine angle medulloblastoma: a retrospective study of 15 cases. Sci Rep 10:9769 (2020). PubMed: 32555264
- Zhou J et al. Dynamic decreased expression and hypermethylation of secreted frizzled-related protein 1 and 4 over the course of pulmonary fibrosis in mice. Life Sci 218:241-252 (2019). PubMed: 30586565
- Robson JP et al. Identification of CD24 as a marker of Patched1 deleted medulloblastoma-initiating neural progenitor cells. PLoS One 14:e0210665 (2019). PubMed: 30657775
- Hawkshaw NJ et al. Deciphering the molecular morphology of the human hair cycle: Wnt signalling during the telogen-anagen transformation. Br J Dermatol N/A:N/A (2019). PubMed: 31314901
- Esteve P et al. Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer's disease pathogenesis. Nat Neurosci 22:1258-1268 (2019). PubMed: 31308530