Anti-SF3B3抗体(ab96683)
Key features and details
- Rabbit polyclonal to SF3B3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SF3B3抗体
参阅全部 SF3B3 一抗 -
描述
兔多克隆抗体to SF3B3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Zebrafish
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免疫原
Synthetic peptide containing a sequence corresponding to a region within amino acids 1154-1217 (PLCGRDHLSF RSYYFPVKNV IDGDLCEQFN SMEPNKQKNV SEELDRTPPE VSKKLEDIRT RYAF) of Human SF3B3 (NP_036558).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96683于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 136 kDa.
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| IHC-P |
1/100 - 1/250.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 136 kDa. |
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IHC-P
1/100 - 1/250. |
靶标
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功能
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. -
序列相似性
Belongs to the RSE1 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 23450 Human
- Entrez Gene: 101943 Mouse
- Entrez Gene: 100334114 Zebrafish
- Entrez Gene: 406824 Zebrafish
- Omim: 605592 Human
- SwissProt: Q15393 Human
- SwissProt: Q921M3 Mouse
- SwissProt: Q1LVE8 Zebrafish
see all -
别名
- KIAA0017 antibody
- Pre mRNA splicing factor SF3b 130 kDa subunit antibody
- Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
see all
图片
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Western blot - Anti-SF3B3 antibody (ab96683)Anti-SF3B3 antibody (ab96683) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 136 kDa
5% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody (ab96683)ab96683, at 1/100 dilution, staining SF3B3 in paraffin-embedded Saos-2 by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab96683 被引用在 6 文献中.
- Zhang S et al. Multi-omics approaches identify SF3B3 and SIRT3 as candidate autophagic regulators and druggable targets in invasive breast carcinoma. Acta Pharm Sin B 11:1227-1245 (2021). PubMed: 34094830
- Chakraborty P et al. DHX9 helicase promotes R-loop formation in cells with impaired RNA splicing. Nat Commun 9:4346 (2018). PubMed: 30341290
- Chen K et al. Alternative Splicing of EZH2 pre-mRNA by SF3B3 Contributes to the Tumorigenic Potential of Renal Cancer. Clin Cancer Res 23:3428-3441 (2017). PubMed: 27879367
- Llorian M et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016). WB ; Mouse . PubMed: 27317697
- Lin-Moshier Y et al. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem 288:355-67 (2013). WB . PubMed: 23148228
- Yang G et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med 210:241-56 (2013). ICC/IF . PubMed: 23359068