重组Anti-SET/TAF-I抗体[EPR12973] (ab181990)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12973] to SET/TAF-I
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SET/TAF-I抗体[EPR12973]
参阅全部 SET/TAF-I 一抗 -
描述
兔单克隆抗体[EPR12973] to SET/TAF-I -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat , 293T, HeLa, HepG2 whole cell lysate (ab7900); Human nephroblastoma and rat spleen tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12973 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181990于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/10000 - 1/100000. Detects a band of approximately 37, 39 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/1000.
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IP |
1/30.
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Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/10000 - 1/100000. Detects a band of approximately 37, 39 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/1000. |
IP
1/30. |
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. -
组织特异性
Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor. -
疾病相关
Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN. -
序列相似性
Belongs to the nucleosome assembly protein (NAP) family. -
结构域
The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity. -
翻译后修饰
Isoform 2 is phosphorylated on Ser-15 and Thr-23.
Isoform 2 is acetylated on Lys-11.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated. -
细胞定位
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 6418 Human
- Entrez Gene: 56086 Mouse
- Entrez Gene: 671392 Mouse
- Entrez Gene: 307947 Rat
- Omim: 600960 Human
- SwissProt: Q01105 Human
- SwissProt: Q9EQU5 Mouse
- SwissProt: Q63945 Rat
see all -
别名
- 2PP2A antibody
- HLA DR associated protein II antibody
- HLA-DR-associated protein II antibody
see all
图片
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All lanes : Anti-SET/TAF-I antibody [EPR12973] (ab181990) at 1/100000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Hela cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDaBlocking/dilution buffer and concentration: 5% NFDM/TBST
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Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SET/TAF-I with Purified ab181990 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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Immunohistochemical analysis of paraffin-embedded Human nephroblastoma tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining SET/TAF-I using ab181990 at 1/1000 dilution. A Dylight 555 conjugated Goat anti rabbit IgG at 1/200 dilution was used for secondary antibody detection. Counter stain: Dapi.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling SET/TAF-I using ab181990at a 1/10 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.
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Immunoprecipitation of Jurkat cell lysate labeling SET/TAF-I using ab181990 at a 1/30 dilution (lane 1). A goat anti-rabbit IgG, (H+L), peroxidase conjugated secondary antibody was used at a dilution of 1/1000. Blocking/dilution buffer and concentration: 5% NFDM/TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab181990 被引用在 9 文献中.
- Zhang Q et al. Genome-wide CRISPR/Cas9 transcriptional activation screen identifies a histone acetyltransferase inhibitor complex as a regulator of HIV-1 integration. Nucleic Acids Res 50:6687-6701 (2022). PubMed: 35713529
- Höllmüller E et al. Site-specific ubiquitylation acts as a regulator of linker histone H1. Nat Commun 12:3497 (2021). PubMed: 34108453
- Yen YT et al. Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response. Nat Commun 12:7297 (2021). PubMed: 34911954
- Mandemaker IK et al. Histone H1 eviction by the histone chaperone SET reduces cell survival following DNA damage. J Cell Sci 133:N/A (2020). PubMed: 32184266
- Liu Y et al. TAF-Iß deficiency inhibits proliferation and promotes apoptosis by rescuing PP2A and inhibiting the AKT/GSK-3ß pathway in leukemic cells. Exp Ther Med 18:3801-3808 (2019). PubMed: 31611934
- Yang Z et al. A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats. Chemosphere 231:151-160 (2019). PubMed: 31129395
- Ma W & Mayr C A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3'UTR-Mediated Protein-Protein Interactions. Cell 175:1492-1506.e19 (2018). PubMed: 30449617
- Ma J et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441
- Berkovits BD & Mayr C Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature 522:363-7 (2015). WB, Flow Cyt . PubMed: 25896326