Anti-SESN3抗体(ab97792)
Key features and details
- Rabbit polyclonal to SESN3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-SESN3抗体 -
描述
兔多克隆抗体to SESN3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 21 and 266 of Human SESN3 (NM_144665).
-
阳性对照
- WB: Neuro-2a, NIH/3T3, RAW 264.7, C2C12, Rat2 cell lysate; ICC/IF: A549 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97792于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
-
组织特异性
Widely expressed. -
序列相似性
Belongs to the sestrin family. - Information by UniProt
-
数据库链接
- Entrez Gene: 143686 Human
- Entrez Gene: 75747 Mouse
- Entrez Gene: 315427 Rat
- Omim: 607768 Human
- SwissProt: P58005 Human
- SwissProt: Q9CYP7 Mouse
- Unigene: 659934 Human
- Unigene: 325126 Mouse
see all -
别名
- MGC29667 antibody
- OTTHUMP00000236979 antibody
- OTTHUMP00000236986 antibody
see all
图片
-
All lanes : Anti-SESN3 antibody (ab97792) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa7.5% SDS-PAGE
Signal was developed with Trident ECL plus-Enhanced.
-
Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cell labeling SESN3 with ab97792 at a 1/200 dilution. The lower image was merged with DNA probe.
-
Anti-SESN3 antibody (ab97792) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa7.5% SDS-PAGE
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab97792 被引用在 9 文献中.
- Salcher S et al. A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma. Oncogene 39:1080-1097 (2020). PubMed: 31591479
- Kim M et al. Sestrins are evolutionarily conserved mediators of exercise benefits. Nat Commun 11:190 (2020). PubMed: 31929512
- Wang Y et al. CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway. J Exp Clin Cancer Res 39:169 (2020). PubMed: 32847606
- Li R et al. Cardioprotective roles of sestrin 1 and sestrin 2 against doxorubicin cardiotoxicity. Am J Physiol Heart Circ Physiol 317:H39-H48 (2019). PubMed: 31026186
- Zeng N et al. Sestrins are differentially expressed with age in the skeletal muscle of men: A cross-sectional analysis. Exp Gerontol 110:23-34 (2018). WB . PubMed: 29751091
- Zeng N et al. Acute resistance exercise induces Sestrin2 phosphorylation and p62 dephosphorylation in human skeletal muscle. Physiol Rep 5:N/A (2017). PubMed: 29263116
- Wang X et al. Sestrin2 and sestrin3 suppress NK-92 cell-mediated cytotoxic activity on ovarian cancer cells through AMPK and mTORC1 signaling. Oncotarget 8:90132-90143 (2017). PubMed: 29163816
- Park HW et al. Hepatoprotective role of Sestrin2 against chronic ER stress. Nat Commun 5:4233 (2014). PubMed: 24947615
- Kang X et al. The inhibitory effect of ethanol on Sestrin3 in the pathogenesis of ethanol-induced liver injury. Am J Physiol Gastrointest Liver Physiol 307:G58-65 (2014). PubMed: 24833709