Key features and details
- Rabbit polyclonal to SESN3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
经测试应用适用于: WB, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Human
Recombinant protein fragment corresponding to a region within amino acids 21 and 266 of Human SESN3 (NM_144665).
- WB: Neuro-2a, NIH/3T3, RAW 264.7, C2C12, Rat2 cell lysate; ICC/IF: A549 cells.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
纯度Immunogen affinity purified
序列相似性Belongs to the sestrin family.
- Information by UniProt
- MGC29667 antibody
- OTTHUMP00000236979 antibody
- OTTHUMP00000236986 antibody
All lanes : Anti-SESN3 antibody (ab97792) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa
Signal was developed with Trident ECL plus-Enhanced.
Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cell labeling SESN3 with ab97792 at a 1/200 dilution. The lower image was merged with DNA probe.
Anti-SESN3 antibody (ab97792) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa
ab97792 被引用在 9 文献中.
- Salcher S et al. A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma. Oncogene 39:1080-1097 (2020). PubMed: 31591479
- Kim M et al. Sestrins are evolutionarily conserved mediators of exercise benefits. Nat Commun 11:190 (2020). PubMed: 31929512
- Wang Y et al. CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway. J Exp Clin Cancer Res 39:169 (2020). PubMed: 32847606
- Li R et al. Cardioprotective roles of sestrin 1 and sestrin 2 against doxorubicin cardiotoxicity. Am J Physiol Heart Circ Physiol 317:H39-H48 (2019). PubMed: 31026186
- Zeng N et al. Sestrins are differentially expressed with age in the skeletal muscle of men: A cross-sectional analysis. Exp Gerontol 110:23-34 (2018). WB . PubMed: 29751091
- Zeng N et al. Acute resistance exercise induces Sestrin2 phosphorylation and p62 dephosphorylation in human skeletal muscle. Physiol Rep 5:N/A (2017). PubMed: 29263116
- Wang X et al. Sestrin2 and sestrin3 suppress NK-92 cell-mediated cytotoxic activity on ovarian cancer cells through AMPK and mTORC1 signaling. Oncotarget 8:90132-90143 (2017). PubMed: 29163816
- Park HW et al. Hepatoprotective role of Sestrin2 against chronic ER stress. Nat Commun 5:4233 (2014). PubMed: 24947615
- Kang X et al. The inhibitory effect of ethanol on Sestrin3 in the pathogenesis of ethanol-induced liver injury. Am J Physiol Gastrointest Liver Physiol 307:G58-65 (2014). PubMed: 24833709