概述

  • 产品名称
    Anti-Serotonin抗体[YC5/45]
    参阅全部 Serotonin 一抗
  • 描述
    大鼠单克隆抗体[YC5/45] to Serotonin
  • 宿主
    Rat
  • 经测试应用
    适用于: IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • 免疫原

    5HT-bovine seroalbumin.

  • 阳性对照
    • In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.
  • 常规说明


    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • 纯度
    Tissue culture supernatant
  • Primary antibody说明
    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
  • 克隆
    单克隆
  • 克隆编号
    YC5/45
  • 同种型
    IgG2c
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 22474435
IHC-Fr Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).

靶标

  • 相关性
    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.
  • 细胞定位
    Cytoplasmic and Secreted
  • 别名
    • 5 hydroxytryptamine antibody
    • 5HT antibody

图片

  • ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.

  • IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Paraformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.

    See Abreview

文献

This product has been referenced in:
  • Sitaraman D  et al. Discrete Serotonin Systems Mediate Memory Enhancement and Escape Latencies after Unpredicted Aversive Experience inDrosophilaPlace Memory. Front Syst Neurosci 11:92 (2017). Read more (PubMed: 29321732) »
  • Dhillon RS  et al. Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy. Acta Neuropathol Commun 4:89 (2016). IHC-Fr . Read more (PubMed: 27552807) »
See all 11 Publications for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Power Block™ Universal Blocking Reagent(Biogenex) as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 37°C

Ms. Szu-yu Liu

Verified customer

提交于 Nov 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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