Anti-Serotonin抗体(ab6444)

Reviews (1)Q&A (1)References (1)

Key features and details

  • Rabbit polyclonal to Serotonin
  • Suitable for: ELISA, ICC
  • Reacts with: Species independent
  • Isotype: IgG

概述

  • 产品名称

    Anti-Serotonin抗体
    参阅全部 Serotonin 一抗

  • 描述

    兔多克隆抗体to Serotonin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, ICCmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule corresponding to Serotonin. Synthetic 5-Hydroxytryptamine conjugated to protein carrier (PC)

  • 常规说明

    ab6444 specific to conjugated 5-Hydroxytryptamine (Serotonin)

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...

  • 纯度

    Ammonium Sulphate Precipitation

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

靶标

  • 相关性

    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

  • 细胞定位

    Cytoplasmic and Secreted

  • 别名

    • 5 hydroxytryptamine antibody
    • 5HT antibody

文献 (1)

发表研究结果有使用 ab6444？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6444 被引用在 1 文献中.

  • Chen Y  et al. Stress inhibits tryptophan hydroxylase expression in a rat model of depression. Oncotarget 8:63247-63257 (2017). PubMed: 28968985

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Serotonin antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
No
Specification
Colon
Blocking step
hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
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Vinicius Kannen Cardoso

Verified customer

提交于 Aug 10 2016

Question

The immunogen is listed asbeing conjugated toan NO2 group. Do you know the position of this conjugated nitro group? Is it conjugated to the N-terminus of the serotonin? Is it nitro-serotonin or does it block the amino group?

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Answer

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

There is a mistake on the datasheet, and the antibody was raised against serotonin conjugated to a protein carrier atthe amino terminus. There was no nitro group in the immunogen. I will have the datasheet updated as soon as possible, and I apologize for this error and any confusion.

Please let me know if you have any questions or if there is anything else that you need and I'll be happy to help further.

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