Anti-SERCA1 ATPase抗体[VE121G9] (ab2819)
![Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-302963-anti-serca1-atpase-antibody-ve121g9-western-blot.jpg "Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)")
Key features and details
- Mouse monoclonal [VE121G9] to SERCA1 ATPase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Rabbit, Dog, Human, Pig, Amphibian
- Isotype: IgG1
概述
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产品名称
Anti-SERCA1 ATPase抗体[VE121G9]
参阅全部 SERCA1 ATPase 一抗 -
描述
小鼠单克隆抗体[VE121G9] to SERCA1 ATPase -
宿主
Mouse -
特异性
Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase. -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Dog, Human, Pig, Amphibian -
免疫原
Full length native protein (purified) corresponding to Rabbit SERCA1 ATPase. Purified rabbit skeletal muscle sarcoplasmic reticulum.
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表位
This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum. -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Ascites -
克隆
单克隆 -
克隆编号
VE121G9 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa). | |
| ICC/IF | 1/500. | |
| IHC-P | 1/200. |
靶标
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功能
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
组织特异性
Skeletal muscle, fast twitch muscle (type II) fibers. -
疾病相关
Brody myopathy -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
发展阶段
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
细胞定位
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Entrez Gene: 100037716 Rabbit
- Entrez Gene: 116601 Rat
- Omim: 108730 Human
- SwissProt: O14983 Human
- SwissProt: Q8R429 Mouse
- SwissProt: P04191 Rabbit
see all -
别名
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
图片
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Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)All lanes : Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/2000 dilution
Lane 1 : Human skeletal muscle.
Lane 2 : Rat skeletal muscle.
Lane 3 : Mouse skeletal muscle
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Developed using the ECL technique.
Predicted band size: 110 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-219066-anti-serca1-atpase-antibody-ve121g9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)Immunocytochemistry/Immunofluorescence analysis of SERCA1 ATPase shows staining in A2058 cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with or ab2819 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse IgG secondary antibody. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-219065-anti-serca1-atpase-antibody-ve121g9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)Immunocytochemistry/Immunofluorescence analysis of SERCA1 ATPase shows staining in HeLa cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with or ab2819 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse IgG secondary antibody. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-219064-anti-serca1-atpase-antibody-ve121g9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)Immunocytochemistry/Immunofluorescence analysis of SERCA1 ATPase shows staining in U251 cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with or ab2819 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse IgG secondary antibody. Images were taken at 60X magnification.
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![Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-6519-anti-serca1-atpase-antibody-ve121g9-western-blot.jpg)
Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/500 dilution + Skeletal Muscle (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The 99 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SERCA1 ATPase. -
![Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-6518-anti-serca1-atpase-antibody-ve121g9-western-blot.jpg)
Western blot - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)This image is courtesy of an anonymous AbreviewAnti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/1000 dilution (in PBS tweeb 0.05% for 1 hour at 22°C) + Whole tissue lysate of human neck muscle. at 20 µg
Secondary
An HRP-conjugated sheep anti-mouse polyclonal at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutes
Blocking Step: 5% milk for 16 hours at 22°C -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-6516-anti-serca1-atpase-antibody-ve121g9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human heart tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing SERCA1 ATPase ab2819 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)](https://www.abcam.cn/ps/products/2/ab2819/Images/ab2819-6515-anti-serca1-atpase-antibody-ve121g9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing SERCA1 ATPase ab2819 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
实验方案
数据表及文件
文献 (20)
ab2819 被引用在 20 文献中.
- Molenaar JP et al. Clinical, morphological and genetic characterization of Brody disease: an international study of 40 patients. Brain 143:452-466 (2020). PubMed: 32040565
- Hadrevi J et al. Calcium Fluxes in Work-Related Muscle Disorder: Implications from a Rat Model. Biomed Res Int 2019:5040818 (2019). PubMed: 31662979
- Ivarsson N et al. SR Ca2+ leak in skeletal muscle fibers acts as an intracellular signal to increase fatigue resistance. J Gen Physiol 151:567-577 (2019). PubMed: 30635368
- Verkerke ARP et al. Phospholipid methylation regulates muscle metabolic rate through Ca2+ transport efficiency. Nat Metab 1:876-885 (2019). PubMed: 32405618
- Elrabie Ahmed M et al. Differential isoform expression of SERCA and myosin heavy chain in hypopharyngeal muscles. Acta Otorhinolaryngol Ital 39:220-229 (2019). PubMed: 30933182
