952x200-hrp

HRP secondary antibodies

Horseradish Peroxidase (HRP) has a high turnover rate that allows HRP secondary antibodies to generate strong signal in a short time span. HRP secondary antibodies are commomly used in western blot (WB), immunohistochemistry (IHC) and ELISA. Efficacy of HRP secondary antibodies is achieved by the optimal number of HRP molecules conjugated per antibody and specificity of the secondary antibody.

No nonspecific binding. No background. Clear bands.(Researcher from Universidade do Porto, Portugal)

Abcam’s anti-rabbit HRP is very reliable and economical to use. We ended up using 10x less than our old antibody from a different company. Since it is so robust, we started calling it the epic rabbit antibody to distinguish it from the old one we have in the lab. (Researcher from University of Massachusetts Medical School, United States).

Goat anti-rabbit secondary antibody- ab205718

The substrates commonly used with HRP secondary antibodies fall into different categories including chromogenic (e.g. DAB, TMB, OPD), fluorogenic (e.g. ADHP) and chemiluminescent (e.g. ECL) substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively.

Our HRP secondary antibodies have been extensively tested and optimized for diverse applications including WB, IHC and ELISA.

Abcam’s HRP secondaries advantages are:

  • High sensitivity – detect low-abundant targets with high signal-to-noise ratios due to an optimal number of HRP molecules per antibody.
  • Robustness – can be used at dilutions ranging from 1/2,000 to 1/20,000 and provide high specificity and minimal background.
  • Stability – they are supplied in a liquid format that is ready-to-use and can be stored at 4°C.
  • Flexibility – compatible with chromogenic, fluorogenic and chemiluminescent substrates.
  • Cost-effective – use less conjugate per assay and perform more blots.


Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 heavy chain (HRP) (ab97240).

A section of human colon formalin fixed paraffin embedded tissue section* was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with anti-Histone H1.0 antibody (ab11080), 7.5µg/ml overnight at +4°C. A Goat Anti-Mouse IgG1 heavy chain (HRP) (ab97240) was used at 1/2000 dilution for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Western blot - Goat Anti-Rabbit HRP (IgG H&L) (ab97051)

HeLa (Human epithelial carcinoma cell line) whole cell lysate (10 µg) was loaded in each lane. All lanes were incubated with anti-beta Actin antibody (ab8227) at 1 µg/ml as the primary antibody. Goat Anti-Rabbit HRP (IgG H&L) (ab97051) was used as the secondary antibody at 1/2000 dilution (lanes 1and 2), 1/10000 dilution (lanes 3 and 4) and 1/20000 dilution (lanes 5 and 6). The blot was developed using the ECL technique and performed under reducing conditions. Exposure time was 10 seconds.

Anti-mouse HRP secondary antibodies

Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.

HostNon-preadsorbed*Preadsorbed*
GoatAnti-mouse HRP (IgG H&L)Yes

Anti-mouse IgG Fc HRPYes

Anti-mouse IgG1 heavy chain HRPYes

Anti-mouse IgG2a heavy chain HRPYes

Anti-mouse IgG2b heavy chain HRPYes

Anti-mouse IgG2c heavy chain HRPYes

Anti-mouse IgG3 heavy chain HRPYes

Anti-mouse IgM mu chain HRPYes

Anti-mouse IgA alpha chain HRPYes

Anti-mouse IgG+IgM HRP (H&L)Yes

Anti-mouse IgG+IgM+IgA HRP (H&L)Yes

F(ab) Anti-mouse HRP (IgG H&L)No

F(ab')2 Anti-mouse HRP (IgG H&L)Yes

F(ab')2 Anti-mouse IgG Fc HRPYes

F(ab')2 Anti-mouse IgG F(ab')2 HRPYes

F(ab')2 Anti-mouse IgM mu chain HRPYes

F(ab')2 Anti-mouse IgG+IgM+IgA HRP (H&L)Yes
RabbitAnti-mouse HRP (IgG H&L)Yes

F(ab')2 Anti-mouse HRP (IgG H&L)Yes
DonkeyAnti-mouse HRP (IgG H&L)Yes

F(ab')2 Anti-mouse HRP (IgG H&L)Yes

* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow through.

Anti-rabbit HRP secondary antibodies

Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.

HostNon-preadsorbed*Preadsorbed*
GoatAnti-Rabbit HRP (IgG H&L)Yes

Anti-Rabbit IgG Fc HRPYes

Anti-Rabbit IgM mu chain HRPYes

Anti-Rabbit IgA alpha chain HRPNo

F(ab) Anti-Rabbit HRP (IgG H&L)No

F(ab')2 Anti-Rabbit HRP (IgG H&L)Yes

F(ab')2 Anti-Rabbit IgG Fc HRPYes

F(ab')2 Anti-Rabbit IgG F(ab')2 HRPYes
DonkeyAnti-Rabbit HRP (IgG H&L)
Yes

F(ab')2 Anti-Rabbit HRP (IgG H&L)Yes
MouseAnti-Rabbit IgG heavy chain HRPNo

Anti-Rabbit IgG light chain HRPNo

Anti-Rabbit IgG+IgM+IgA heavy chain HRPNo

* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow through.

Anti-rat HRP secondary antibodies

Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.

HostNon-preadsorbed*Preadsorbed*
GoatAnti-Rat HRP (IgG H&L)Yes

Anti-Rat IgG Fc HRPYes

Anti-Rat IgM mu chain HRPYes

Anti-Rat IgA alpha chain HRPYes

Anti-Rat IgG+IgM HRP (H&L)Yes

F(ab) Anti-Rat HRP (IgG H&L)No

F(ab')2 Anti-Rat HRP (IgG H&L)Yes

F(ab')2 Anti-Rat IgG Fc HRPYes

F(ab')2 Anti-Rat IgG F(ab')2 HRPYes
RabbitAnti-Rat IgG+IgM+IgA HRP (H&L)No

F(ab')2 Anti-Rat HRP (IgG H&L)Yes
DonkeyAnti-Rat HRP (IgG H&L)Yes

F(ab')2 Anti-Rat HRP (IgG H&L)Yes
MouseAnti-Rat HRP (IgG H&L)No

Anti-Rat IgG1 HRP (H&L)No

Anti-Rat IgG1 heavy chain HRPNo

Anti-Rat IgG1 gamma chain HRPNo

Anti-Rat IgG2a HRP (H&L)No

Anti-Rat IgG2a heavy chain HRPNo

Anti-Rat IgG2b HRP (H&L)No

Anti-Rat IgG2b gamma chain HRPNo

Anti-Rat IgG2c HRP (H&L)No

Anti-Rat IgG2c heavy chain HRPNo

Anti-Rat IgM mu chain HRPNo

Anti-Rat IgG+IgM light chain HRPNo

Anti-Rat kappa light chain HRPNo

* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow through.

Anti-goat HRP secondary antibodies

Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.

HostNon-preadsorbed*Preadsorbed*
RabbitAnti-goat HRP (IgG H&L)Yes

Anti-goat IgG Fc HRPYes

Anti-goat IgM HRP (H&L)No

Anti-goat IgA HRP (H&L)No

Anti-goat IgG+IgM+IgA HRP (H&L)No

F(ab')2 Anti-goat HRP (IgG H&L)Yes
DonkeyAnti-goat HRP (IgG H&L)Yes

F(ab) Anti-goat HRP (IgG H&L)No

F(ab')2 Anti-goat HRP (IgG H&L)Yes
MouseAnti-goat IgG Fc HRPYes

* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow through.

Anti-chicken HRP secondary antibodies

Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.

HostNon-preadsorbed*Preadsorbed*
GoatAnti-Chicken IgM mu chain HRPNo

Anti-Chicken IgA HRP (H&L)No

Anti-Chicken HRP (IgY H&L)Yes

Anti-Chicken IgY Fc HRPNo
RabbitAnti-Chicken HRP (IgY H&L)No

F(ab')2 Anti-Chicken HRP (IgY H&L)No

* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow through.

Anti-human HRP secondary antibodies

Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.

HostNon-preadsorbed*Preadsorbed*
GoatAnti-Human HRP (IgG H&L)Yes

Anti-Human IgG F(ab')2 HRPNo

Anti-Human IgM mu chain HRPYes


Anti-Human IgA alpha chain HRP
Yes

Anti-Human IgG+IgM HRP (H&L)No

Anti-Human IgG+IgM+IgA HRP (H&L)No

F(ab')2 Anti-Human HRP (IgG H&L)Yes

F(ab')2 Anti-Human IgG Fc HRPYes

F(ab')2 Anti-Human IgG F(ab')2 HRPYes

F(ab')2 Anti-Human IgM mu chain HRPNo
RabbitAnti-Human HRP (IgG H&L)Yes

Anti-Human IgM mu chain HRPNo

Anti-Human IgA HRP (H&L)No

Anti-Human IgA alpha chain HRPNo

F(ab')2 Anti-Human HRP (IgG H&L)Yes
DonkeyAnti-Human HRP (IgG H&L)Yes

F(ab')2 Anti-Human HRP (IgG H&L)Yes
MouseAnti-Human IgG Fc HRPNo

Anti-Human IgG hinge heavy chain HRPNo

Anti-Human IgG2 Fc HRPNo

Anti-Human IgG2 Fd gamma HRPNo

Anti-Human IgG3 hinge heavy chain HRPNo

Anti-Human IgG4 Fc HRPNo

Anti-Human IgM mu chain HRPNo

Anti-Human IgA1 Fc HRPNo

Anti-Human IgA2 Fc HRPNo

Anti-Human IgD delta chain HRPNo

Anti-Human IgE Fc HRPNo

Anti-Human lambda light chain HRPNo

* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow through.

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