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View our fluorochrome chart, a quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
Tandem fluorescent dyes are conjugated 'dual' fluorescent molecules, for example PE-Cy5. On the antibody, they will be close enough so that the energy can be transferred between the two. The laser excitation wavelength used will excite the donor molecule only (eg PE) – it will not be the correct wavelength to excite the acceptor molecule. The energy then released from the donor molecule will be at the correct wavelength to excite the electron in the acceptor molecule . The acceptor molecule will then release energy in the form of a photon at its own signature wavelength.
So, for example, PE-Cy5 will excite at the excitation wavelength for PE (565 nm) and emit at the emission wavelength for Cy5 (670 nm).
Whether you have to select one or more fluorochromes, we have put together a new fluorochrome chart to make the process quick and easy. It contains aligned emission and excitation spectra for 30 of the most commonly-used fluorochromes with information about popular instrument lasers and filters depicted across the chart for easy visualization. We have included a step-by-step guide to walk you through the process.