重组Anti-SEC61A抗体[EPR14379] (ab183046)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14379] to SEC61A
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SEC61A抗体[EPR14379]
参阅全部 SEC61A 一抗 -
描述
兔单克隆抗体[EPR14379] to SEC61A -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal brain, Daudi and A431 lysates; Human Infiltrating duct carcinoma of breast tissue; A431 and Daudi cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14379 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/40 - 1/60.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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|
WB |
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).
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说明 |
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IP
1/40 - 1/60. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa). |
靶标
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功能
Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins. -
序列相似性
Belongs to the secY/SEC61-alpha family. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 29927 Human
- Entrez Gene: 53421 Mouse
- Entrez Gene: 80843 Rat
- Omim: 609213 Human
- SwissProt: P61619 Human
- SwissProt: P61620 Mouse
- SwissProt: P61621 Rat
- Unigene: 518236 Human
see all -
别名
- chunp6898 antibody
- fb62c11 antibody
- HSEC61 antibody
see all
图片
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All lanes : Anti-SEC61A antibody [EPR14379] (ab183046) at 1/5000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Daudi cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted? -
Western blot analysis of SEC61A in Daudi cell lysate immunoprecipitated using ab183046 at 1/50 dilution (Lane 1). Lane 2: Negative control.
Secondary antibody: Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (12)
ab183046 被引用在 12 文献中.
- Tranter D et al. Coibamide A Targets Sec61 to Prevent Biogenesis of Secretory and Membrane Proteins. ACS Chem Biol 15:2125-2136 (2020). PubMed: 32608972
- Guo L et al. Leucine improves a-amylase secretion through the general secretory signaling pathway in pancreatic acinar cells of dairy calves. Am J Physiol Cell Physiol 318:C1284-C1293 (2020). PubMed: 32320287
- Van Nieuwenhove E et al. Defective Sec61a1 underlies a novel cause of autosomal dominant severe congenital neutropenia. J Allergy Clin Immunol 146:1180-1193 (2020). PubMed: 32325141
- Tian S et al. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep 28:2517-2526.e5 (2019). PubMed: 31484065
- Yau WL et al. Model System for the Formation of Tick-Borne Encephalitis Virus Replication Compartments without Viral RNA Replication. J Virol 93:N/A (2019). PubMed: 31243132