Anti-SEC23A抗体(ab99552)
Key features and details
- Goat polyclonal to SEC23A
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-SEC23A抗体
参阅全部 SEC23A 一抗 -
描述
山羊多克隆抗体to SEC23A -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Horse, Cow, Dog, Human -
免疫原
Synthetic peptide corresponding to Human SEC23A aa 335-346 (internal sequence).
Sequence:C-ANRAATTGHVID
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阳性对照
- NIH 3T3 lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab99552 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99552于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. -
疾病相关
Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects. -
序列相似性
Belongs to the SEC23/SEC24 family. SEC23 subfamily. -
细胞定位
Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 10484 Human
- Entrez Gene: 20334 Mouse
- Entrez Gene: 58817 Rat
- Omim: 610511 Human
- SwissProt: Q15436 Human
- SwissProt: Q01405 Mouse
- Unigene: 272927 Human
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别名
- CLSD antibody
- Protein transport protein Sec23A antibody
- SC23A_HUMAN antibody
see all
图片
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Anti-SEC23A antibody (ab99552) at 2 µg/ml (incubation time 1 hour) + NIH 3T3 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (2)
ab99552 被引用在 2 文献中.
- Cao Q et al. Collagen VII maintains proteostasis in dermal fibroblasts by scaffolding TANGO1 cargo. Matrix Biol 111:226-244 (2022). PubMed: 35779741
- Dieterich IA et al. Acetyl-CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway. Sci Rep 11:2013 (2021). PubMed: 33479349