Application
Western blot
Sample
Mouse Cell lysate - whole cell (Germline stem cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
Germline stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 4% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-mouse (santa cruz #sc-2005)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 28 kDa
Additional data
Additional Notes
duplicate samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Neha Garg
Verified customer
提交于 Jul 26 2019