Name: Anti-SDHA antibody [EPR9043(B)]
Brand: RabMAb
SKU: ab137040
Rating: 4 (3 reviews)

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Western blot - Anti-SDHA antibody [EPR9043(B)] (ab137040)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9043(B)] to SDHA
Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

产品名称

Anti-SDHA抗体[EPR9043(B)]
参阅全部 SDHA 一抗
描述

兔单克隆抗体[EPR9043(B)] to SDHA
宿主

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

免疫原

Synthetic peptide within Human SDHA aa 550-650. The exact sequence is proprietary.
阳性对照
- WB: Wild-type HEK-293 whole cell lysate. MCF7, HT1080, Jurkat and HepG2 whole cell lysate. Mouse brain and kidney tissue lysate. Rat brain tissue lysate. IHC-P: Human, mouse and rat brain tissue. ICC: HeLa cells. IP: Jurkat and HeLa cell lysate. Flow Cyt: HeLa cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR9043(B)
同种型

IgG
研究领域

Alternative Versions
- Anti-SDHA antibody [EPR9043(B)] - BSA and Azide free (ab240098)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab7899)
Recombinant Protein
- Recombinant Human SDHA protein (Tagged) (ab226268)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

应用	Ab评论	说明
WB	(2)	1/1000 - 1/5000. Predicted molecular weight: 72 kDa. Unpurified dilution 1/1000 - 1/10000.
IP		1/10 - 1/20. Unpurified dilution 1/10 - 1/100.
Flow Cyt		1/10 - 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Unpurified dilution 1/10 - 1/100.
IHC-P	(1)	1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.

说明
WB 1/1000 - 1/5000. Predicted molecular weight: 72 kDa. Unpurified dilution 1/1000 - 1/10000.
IP 1/10 - 1/20. Unpurified dilution 1/10 - 1/100.
Flow Cyt 1/10 - 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Unpurified dilution 1/10 - 1/100.
IHC-P 1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

功能

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
通路

Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
疾病相关

Defects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
序列相似性

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
细胞定位

Mitochondrion inner membrane.
Target information above from: UniProt accession P31040 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 6389 Human
- Entrez Gene: 66945 Mouse
- Entrez Gene: 157074 Rat
- Omim: 600857 Human
- SwissProt: P31040 Human
- SwissProt: Q8K2B3 Mouse
- SwissProt: Q920L2 Rat
- Unigene: 440475 Human
- Unigene: 158231 Mouse
- Unigene: 101725 Rat
see all
别名
- CMD1GG antibody
- DHSA_HUMAN antibody
- Flavoprotein subunit of complex II antibody
- Fp antibody
- PGL5 antibody
- SDH 2 antibody
- SDH1 antibody
- SDH2 antibody
- SDHA antibody
- SDHF antibody
- Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody
- Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody
- Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody
- Succinate dehydrogenase complex flavoprotein subunit A antibody
- Succinate dehydrogenase complex flavoprotein subunit antibody
- Succinate dehydrogenase complex flavoprotein subunit precursor antibody
- Succinate dehydrogenase complex subunit A antibody
- Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody
- Succinate dehydrogenase complex subunit A flavoprotein antibody
see all

Western blot - Anti-SDHA antibody [EPR9043(B)] (ab137040)

All lanes : Anti-SDHA antibody [EPR9043(B)] (ab137040) at 1/1000 dilution

Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : SDHA knockout HEK-293 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 72 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab137040 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab137040 was shown to specifically react with SDHA in wild-type HEK-293 cells as signal was lost in SDHA knockout cells. Wild-type and SDHA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab137040 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SDHA antibody [EPR9043(B)] (ab137040)

All lanes : Anti-SDHA antibody [EPR9043(B)] (ab137040) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HT1080 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 72 kDa
Western blot - Anti-SDHA antibody [EPR9043(B)] (ab137040)

All lanes : Anti-SDHA antibody [EPR9043(B)] (ab137040) at 1/5000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysate
Lane 3 : Jurkat (human acute T cell leukemia) whole cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse kidney tissue lysate
Lane 6 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 72 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.

Blocking buffer: 5% NFDM /TBST

Diluting buffer: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9043(B)] (ab137040)

ab137040 staining SDHA in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9043(B)] (ab137040)

ab137040 staining SDHA in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9043(B)] (ab137040)

ab137040 staining SDHA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9043(B)] (ab137040)

Immunohistochemichal analysis of paraffin embedded human kidney tissue labelling SDHA with ab137040 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [EPR9043(B)] (ab137040)

Immunohistochemichal analysis of paraffin embedded human testis tissue labelling SDHA with ab137040 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [EPR9043(B)] (ab137040)

Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labelling SDHA with ab137040 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SDHA antibody [EPR9043(B)] (ab137040)

ab137040 staining SDHA in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab137040 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
Immunoprecipitation - Anti-SDHA antibody [EPR9043(B)] (ab137040)

ab137040 immunoprecipitating SDHA. 10µg of Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1: Jurkat whole cell lysate 10ug
Lane 2: ab137040 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab137040 in Jurkat whole cell lysate
Immunoprecipitation - Anti-SDHA antibody [EPR9043(B)] (ab137040)

ab137040 immunoprecipitating SDHA. 10µg of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1: HeLa whole cell lysate (10ug)
Lane 2: ab137040 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab137040 in HeLa whole cell lysate
Flow Cytometry - Anti-SDHA antibody [EPR9043(B)] (ab137040)

ab137040 staining SDHA in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Anti-SDHA antibody [EPR9043(B)] (ab137040)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab137040？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab137040 被引用在 10 文献中.

Li B et al. Mitochondrial-Derived Vesicles Protect Cardiomyocytes Against Hypoxic Damage. Front Cell Dev Biol 8:214 (2020). PubMed: 32426351
Liu Z et al. IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stress. J Exp Clin Cancer Res 39:144 (2020). PubMed: 32727517
Liu L et al. Exogenous nicotinamide adenine dinucleotide administration alleviates ischemia/reperfusion-induced oxidative injury in isolated rat hearts via Sirt5-SDH-succinate pathway. Eur J Pharmacol 858:172520 (2019). PubMed: 31278893
Meléndez-Rodríguez F et al. HIF1a Suppresses Tumor Cell Proliferation through Inhibition of Aspartate Biosynthesis. Cell Rep 26:2257-2265.e4 (2019). PubMed: 30811976
Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233
Li H et al. Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts. Sci Rep 8:5118 (2018). PubMed: 29572489
Bao Y et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). PubMed: 28008780
Chen XF et al. Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats. Nutr Diabetes 8:1 (2018). PubMed: 29330446
Riedl I et al. AMPK?3 is dispensable for skeletal muscle hypertrophy induced by functional overload. Am J Physiol Endocrinol Metab 310:E461-72 (2016). PubMed: 26758685
Zecchini V et al. Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer. EMBO J 33:1365-82 (2014). WB, ICC/IF . PubMed: 24837709

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Specification

brain

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Oct 01 2019

Application

Western blot

Sample

Mouse Tissue lysate - other (Mitochondria isolated from mouse brain)

Gel Running Conditions

Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol, 10 min at +55C denaturation. Immobilon-FL transfer membranes (Millipore), overnight transfer at +4C.)

Loading amount

15 µg

Specification

Mitochondria isolated from mouse brain

Blocking step

SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Oct 17 2018

Application

Western blot

Loading amount

150 µg

Gel Running Conditions

Reduced Denaturing

Sample

Saccharomyces cerevisiae Cell lysate - whole cell (Yeast whole cell)

Specification

Yeast whole cell

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

提交于 Dec 09 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

概述

产品名称

描述

宿主

Tested Applications & Species

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

通路

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (10)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDHA antibody [EPR9043(B)]

Western blot abreview for Anti-SDHA antibody [EPR9043(B)]

Western blot abreview for Anti-SDHA antibody [EPR9043(B)]