Anti-SCDGFB/PDGF-D抗体(ab234666)
Key features and details
- Rabbit polyclonal to SCDGFB/PDGF-D
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SCDGFB/PDGF-D抗体
参阅全部 SCDGFB/PDGF-D 一抗 -
描述
兔多克隆抗体to SCDGFB/PDGF-D -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Mouse SCDGFB/PDGF-D aa 1 to the C-terminus. Full length mature chain without signal peptide.
Database link: Q925I7-1 -
阳性对照
- WB: A549 and HeLa whole cell lysate. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab234666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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说明 |
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ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
靶标
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功能
Potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. Activated by proteolytic cleavage and this active form acts as a specific ligand for beta platelet-derived growth factor receptor. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. -
组织特异性
Expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen and colon. In the kidney, expressed by the visceral epithelial cells of the glomeruli. A widespread expression is also seen in the medial smooth muscle cells of arteries and arterioles, as well as in smooth muscle cells of vasa rectae in the medullary area. Expressed in the adventitial connective tissue surrounding the suprarenal artery. In chronic obstructive nephropathy, a persistent expression is seen in glomerular visceral epithelial cells and vascular smooth muscle cells, as well as de novo expression by periglomerular interstitial cells and by some neointimal cells of arteriosclerotic vessels. Expression in normal prostate is seen preferentially in the mesenchyme of the gland while expression is increased and more profuse in prostate carcinoma. Expressed in many ovarian, lung, renal and brain cancer-derived cell lines. -
序列相似性
Belongs to the PDGF/VEGF growth factor family.
Contains 1 CUB domain. -
发展阶段
Not detectable in the earliest stages of glomerulogenesis, and not detected in the metanephric blastema or surrounding cortical interstitial cells. In later stages of glomerulogenesis, localized to epithelial cells transitioning from the early developing nephrons of the comma- and S-shaped stages to the visceral epithelial cells of differentiated glomeruli. In the developing pelvis, expressed at the basement membrane of immature collecting ducts and by presumptive fibroblastic cells in the interstitium. -
翻译后修饰
Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after Arg-247 or Arg-249 by urokinase plasminogen activator gives rise to the active form. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 80310 Human
- Entrez Gene: 71785 Mouse
- Entrez Gene: 66018 Rat
- Omim: 609673 Human
- SwissProt: Q9GZP0 Human
- SwissProt: Q925I7 Mouse
- SwissProt: Q9EQT1 Rat
- Unigene: 352298 Human
see all -
别名
- IEGF antibody
- Iris expressed growth factor antibody
- Iris-expressed growth factor antibody
see all
图片
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All lanes : Anti-SCDGFB/PDGF-D antibody (ab234666) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SCDGFB/PDGF-D using ab234666 at a dilution of 1/100 in ICC/IF. Secondary antibody is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab234666 被引用在 1 文献中.
- Kim MS et al. Potential Role of PDGFRß-Associated THBS4 in Colorectal Cancer Development. Cancers (Basel) 12:N/A (2020). PubMed: 32899998