Anti-SCAMP1抗体(ab3430)
Key features and details
- Rabbit polyclonal to SCAMP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SCAMP1抗体 -
描述
兔多克隆抗体to SCAMP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig
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免疫原
Synthetic peptide corresponding to Human SCAMP1 aa 1-17.
Sequence:MSDFDSNPFADPDLNNP
(Peptide available asab4969) -
阳性对照
- WB: U-87MG, Neuro-2a, SH-SY5Y, SK-N-AS, T98G, IMR-32 and Rat brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).
By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa). By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate. |
靶标
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功能
Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. -
组织特异性
Widely expressed, with highest expression in brain. -
序列相似性
Belongs to the SCAMP family. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9522 Human
- Entrez Gene: 107767 Mouse
- Entrez Gene: 397477 Pig
- Entrez Gene: 29521 Rat
- Omim: 606911 Human
- SwissProt: O15126 Human
- SwissProt: Q8K021 Mouse
- SwissProt: O77735 Pig
see all -
别名
- SCAM1_HUMAN antibody
- SCAMP 1 antibody
- SCAMP 37 antibody
see all
图片
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Western blot - Anti-SCAMP1 antibody (ab3430)All lanes : Anti-SCAMP1 antibody (ab3430) at 2 µg/ml
Lane 1 : U-87MG (Human primary glioblastoma cell line) lysate
Lane 2 : Neuro-2a (Mouse brain) lysate
Lane 3 : SH-SY5Y (Human derived cell line) lysate
Lane 4 : SK-N-AS (Human neuroblastoma cell line) lysate
Lane 5 : T98G (Glioblastoma cell line) lysate
Lane 6 : IMR-32 (Human neuroblastoma cell line) lysate
Lane 7 : Rat brain tissue
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 38 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Blocking buffer: 5% Milk.
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Western blot - Anti-SCAMP1 antibody (ab3430)Western blot detection of SCAMP1 on rat brain extract using ab3430.
数据表及文件
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Datasheet download
文献 (2)
ab3430 被引用在 2 文献中.
- Gerlini R et al. Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice. Cell Death Dis 10:747 (2019). PubMed: 31582725
- Jain P et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). WB, Dot blot ; Hamster . PubMed: 17897946
