Anti-SCAMP1抗体(ab3430)

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Western blot - Anti-SCAMP1 antibody (ab3430)
  • Western blot - Anti-SCAMP1 antibody (ab3430)

Key features and details

  • Rabbit polyclonal to SCAMP1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-SCAMP1抗体

  • 描述

    兔多克隆抗体to SCAMP1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Pig

  • 免疫原

    Synthetic peptide corresponding to Human SCAMP1 aa 1-17.
    Sequence:

    MSDFDSNPFADPDLNNP


    (Peptide available as ab4969)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: U-87MG, Neuro-2a, SH-SY5Y, SK-N-AS, T98G, IMR-32 and Rat brain tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3430于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.
说明
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.

靶标

图片

  • Western blot - Anti-SCAMP1 antibody (ab3430)
    Western blot - Anti-SCAMP1 antibody (ab3430)
    All lanes : Anti-SCAMP1 antibody (ab3430) at 2 µg/ml

    Lane 1 : U-87MG (Human primary glioblastoma cell line) lysate
    Lane 2 : Neuro-2a (Mouse brain) lysate
    Lane 3 : SH-SY5Y (Human derived cell line) lysate
    Lane 4 : SK-N-AS (Human neuroblastoma cell line) lysate
    Lane 5 : T98G (Glioblastoma cell line) lysate
    Lane 6 : IMR-32 (Human neuroblastoma cell line) lysate
    Lane 7 : Rat brain tissue

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution

    Predicted band size: 38 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% Milk.

  • Western blot - Anti-SCAMP1 antibody (ab3430)
    Western blot - Anti-SCAMP1 antibody (ab3430)

    Western blot detection of SCAMP1 on rat brain extract using ab3430.

     

文献 (2)

发表研究结果有使用 ab3430？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3430 被引用在 2 文献中.

  • Gerlini R  et al. Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice. Cell Death Dis 10:747 (2019). PubMed: 31582725
  • Jain P  et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). WB, Dot blot ; Hamster . PubMed: 17897946

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