Anti-SATB1 + SATB2抗体[SATBA4B10] - C-terminal (ab51502)
Key features and details
- Mouse monoclonal [SATBA4B10] to SATB1 + SATB2 - C-terminal
- Suitable for: ICC, IP, WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-SATB1 + SATB2抗体[SATBA4B10] - C-terminal -
描述
小鼠单克隆抗体[SATBA4B10] to SATB1 + SATB2 - C-terminal -
宿主
Mouse -
经测试应用
适用于: ICC, IP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human SATB2 (C terminal).
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阳性对照
- ICC: HT10180 cells. WB: NIH/3T3 and HT1080 whole cell lysate. IP: SATB2 IP in HeLa cell lysate.
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常规说明
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
SATBA4B10 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 2 - 100 µg/ml.
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IP |
Use at an assay dependent concentration.
100-500 µg/sample |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 81 kDa.
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说明 |
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ICC
Use a concentration of 2 - 100 µg/ml. |
IP
Use at an assay dependent concentration. 100-500 µg/sample |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 81 kDa. |
靶标
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细胞定位
SATB1: Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies. SATB2: Nucleus matrix. -
数据库链接
- Entrez Gene: 23314 Human
- Entrez Gene: 6304 Human
- Entrez Gene: 20230 Mouse
- Entrez Gene: 212712 Mouse
- Omim: 602075 Human
- Omim: 608148 Human
- SwissProt: Q01826 Human
- SwissProt: Q9UPW6 Human
see all -
形式
SATB1: There are 2 isoforms produced by alternative splicing.
图片
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All lanes : Anti-SATB1 + SATB2 antibody [SATBA4B10] - C-terminal (ab51502) at 2 µg
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SATB2 knockout HAP1 cell lysate
Lane 3 : Mouse E18 Embyonic brain cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : HT1080 cell lysate
Lane 6 : Saos-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SATB1 + SATB2 antibody [SATBA4B10] - C-terminal staining at 2 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab51502 was shown to bind specifically to SATB1. A band was observed at 100 kDa in wild-type HAP1 cell lysates with no signal observed at this size in SATB1 knockout cell line.
To generate this image, wild-type and SATB1 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-SATB1 + SATB2 antibody [SATBA4B10] - C-terminal (ab51502) at 2 µg/ml
Lane 1 : Wild-type HAP1 cell lysate at 20 µg
Lane 2 : SATB2 knockout HAP1 cell lysate at 20 µg
Lane 3 : Mouse E18 Embyonic brain cell lysate at 20 µg
Lane 4 : NIH/3T3 cell lysate at 20 µg
Lane 5 : HT1080 cell lysate at 20 µg
Lane 6 : Saos-2 cell lysate at 20 µg
Lanes 7 & 9 : Empty at 0 µg
Lane 8 : SATB1 Recombinant Protein cell lysate at 0.1 µg
Lane 10 : SATB2 Recombinant Protein (ab132405) cell lysate at 0.1 µg
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SATB1 + SATB2 antibody [SATBA4B10] - C-terminal staining at 2 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab51502 was shown to bind specifically to SATB1. A band was observed at 100 kDa in wild-type HAP1 cell lysates with no signal observed at this size in SATB1 knockout cell line. To generate this image, wild-type and SATB1 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution
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All lanes : Anti-SATB1 + SATB2 antibody [SATBA4B10] - C-terminal (ab51502) at 2 µg/ml
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SATB2 knockout HAP1 cell lysate
Lane 3 : Mouse E18 Embyonic brain cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : HT1080 cell lysate
Lane 6 : Saos-2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SATB1 + SATB2 antibody [SATBA4B10] - C-terminal staining at 2 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab51502 was shown to bind specifically to SATB1. A band was observed at 100 kDa in wild-type HAP1 cell lysates with no signal observed at this size in SATB1 knockout cell line. To generate this image, wild-type and SATB1 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution
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All lanes : Anti-SATB1 + SATB2 antibody [SATBA4B10] - C-terminal (ab51502) at 2 µg/ml
Lane 1 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 81 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted? -
Immunoprecipitation using ab51502 at 500 µg/sample.
Sample: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared in RIPA buffer.
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4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cell line) cells labeled for SATB2 using ab51502 at 100 µg/ml in immunocytochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (269)
ab51502 被引用在 269 文献中.
- Salamon I et al. mRNA-Decapping Associated DcpS Enzyme Controls Critical Steps of Neuronal Development. Cereb Cortex 32:1494-1507 (2022). PubMed: 34467373
- Han X et al. Loss of RNA-Binding Protein HuR Leads to Defective Ependymal Cells and Hydrocephalus. J Neurosci 42:202-219 (2022). PubMed: 34815315
- Wang W et al. The human FOXM1 homolog promotes basal progenitor cell proliferation and cortical folding in mouse. EMBO Rep 23:e53602 (2022). PubMed: 34935271
- Iwasawa E et al. The Anti-Inflammatory Agent Bindarit Attenuates the Impairment of Neural Development through Suppression of Microglial Activation in a Neonatal Hydrocephalus Mouse Model. J Neurosci 42:1820-1844 (2022). PubMed: 34992132
- Miura Y et al. Engineering brain assembloids to interrogate human neural circuits. Nat Protoc 17:15-35 (2022). PubMed: 34992269