Salmon Sperm ssDNA (ab229278)
概述
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产品名称
Salmon Sperm ssDNA -
常规说明
This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency.
Background: Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from salmon testes DNA by mechanical shearing and heat denaturation to an average size of 100 to 200 base pairs. To reverse any renaturation occurring during storage, this material should be briefly boiled and rapidly chilled prior to use. >75% native nucleic acid.
Purity/Specifity: A260/A280: 1.85 % Hyperchromocity: 30.5.
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经测试应用
适用于: ChIP, In situ hybridization, Blockingmore details
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.16% Tris HCl, 0.03% EDTA -
Concentration information loading...
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研究领域
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相关性
The cell death process during apoptosis is essential for normal tissue homeostasis and cellular differentiation in multi cellular organisms. Single stranded DNA (ss DNA) is produced by nuclear fragmentation during the early stages of the apoptotic process. -
细胞定位
Nuclear -
别名
- Single stranded deoxyribose nucleic acid
- Single stranded DNA
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab229278于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP |
Use at an assay dependent concentration.
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In situ hybridization |
Use at an assay dependent concentration.
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Blocking |
Use at an assay dependent concentration.
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说明 |
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ChIP
Use at an assay dependent concentration. |
In situ hybridization
Use at an assay dependent concentration. |
Blocking
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab229278 被引用在 1 文献中.
- Hu KH et al. ZipSeq: barcoding for real-time mapping of single cell transcriptomes. Nat Methods 17:833-843 (2020). PubMed: 32632238